BCL2L1 抗体 (AA 3-14)
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Quick Overview for BCL2L1 抗体 (AA 3-14) (ABIN3025808)
抗原
See all BCL2L1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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抗原表位
- AA 3-14
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原理
- Bcl-X Antibody Cocktail (azide and preservative free)
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序列
- QSNRELVVDF LS
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纯化方法
- Protein G affinity chromatography
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免疫原
- Amino acids 3-14 (QSNRELVVDFLS) from the human protein were used as the immunogen for this Bcl-X antibody.
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亚型
- IgG2a, kappa
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应用备注
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The optimal dilution of the Bcl-X antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Bcl-X antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- BCL2L1 (BCL2-Like 1 (BCL2L1))
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别名
- Bcl-X
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背景
- Recognizes a protein of 27 kDa, identified as the Bcl-X protein. This mAb cocktail shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins.
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UniProt
- Q07817
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途径
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
抗原
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