BCL2L1 抗体 (AA 3-14)
Quick Overview for BCL2L1 抗体 (AA 3-14) (ABIN3025808)
抗原
See all BCL2L1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 3-14
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纯化方法
- Protein G affinity chromatography
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免疫原
- Amino acids 3-14 (QSNRELVVDFLS) from the human protein were used as the immunogen for this Bcl-X antibody.
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亚型
- IgG2a
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应用备注
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The optimal dilution of the Bcl-X antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Bcl-X antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- BCL2L1 (BCL2-Like 1 (BCL2L1))
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别名
- Bcl-X
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背景
- Recognizes a protein of 27 kDa, identified as the Bcl-X protein. This mAb cocktail shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This mAb reacts with both Bcl-XS and Bcl-XL proteins.
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途径
- Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
抗原
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