BAX 抗体 (AA 3-16)
Quick Overview for BAX 抗体 (AA 3-16) (ABIN3025801)
抗原
See all BAX 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 3-16
-
无交叉反应
- 小鼠, 大鼠
-
纯化方法
- Protein G affinity chromatography
-
免疫原
- Amino acids 3-16 (GSGEQPRGGGPTSS) from the human protein were used as the immunogen for this anti-Bax antibody.
-
亚型
- IgG1
-
-
anti-BCL2-Associated X Protein (BAX) (AA 84-175) antibody
BAX 适用: 人, 小鼠, 大鼠, Pig, Cow, 兔, 小鸡, 斑马鱼 WB, ELISA, FACS, IP, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Associated X Protein (BAX) (AA 1-100) antibodyBAX 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Associated X Protein (BAX) (N-Term) antibodyBAX 适用: 人, 小鼠, 大鼠, 猴, Pig, Cow, 兔, 犬, 小鸡, 绵羊, 马, 豚鼠, 山羊, Hamster, Avian, Cat, 驴 WB, IF 宿主: 山羊 Polyclonal unconjugated
anti-BCL2-Associated X Protein (BAX) (AA 1-158) antibodyBAX 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-BCL2-Associated X Protein (BAX) (AA 1-192) antibodyBAX 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 1F5-1B7 unconjugated
anti-BCL2-Associated X Protein (BAX) (AA 13-160) antibodyBAX 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 4H9B11 unconjugated
anti-BCL2-Associated X Protein (BAX) antibodyBAX 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 2G6B2 unconjugated
anti-BCL2-Associated X Protein (BAX) antibodyBAX 适用: 人 IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 2D8H2 unconjugated
anti-BCL2-Associated X Protein (BAX) (AA 41-76) antibodyBAX 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB0952 unconjugated
anti-BCL2-Associated X Protein (BAX) (N-Term) antibodyBAX 适用: 人, Pig, Cow, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
The optimal dilution of the anti-Bax antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
-
-
-
浓度
- 1 mg/mL
-
缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
-
储存液
- Azide free
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store the anti-Bax antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
-
-
- BAX (BCL2-Associated X Protein (BAX))
-
别名
- Bax
-
背景
- Recognizes a protein of 21 kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
-
途径
- p53 Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
抗原
-
