Immunohistochemistry on paraffin sections (10 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS containing 0.09 % sodium azide as preservative
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.
Most of dual-specificity protein phosphatases (DSPs) play an important role in the regulation of mitogenic signal transduction and controlling the cell cycle in response to extracellular stimuli . DUSP23 has been shown to dephosphorylate p44ERK1 but not p38 and p54SAPKbeta in vitro. Reverse transcription-PCR (RT-PCR) revealed that the DUSP23 was expressed in most fetal tissues and two adult tissues: testis and colon.Synonyms: Dual specificity protein phosphatase 23, LDP3, Low molecular mass dual specificity phosphatase 3, VH1-like phosphatase Z, VHZ