Histone 抗体
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- 抗原
- Histone
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适用
- 人, 大鼠, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (IF)
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone antibody.
- 克隆位点
- AE-4
- 亚型
- IgG2a kappa
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anti-Histone antibody
适用: 人, 大鼠, 小鼠 WB, IHC (p), FACS, IF 宿主: 小鼠 Monoclonal AE-4 unconjugated
anti-Histone antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Optimal dilution of the Histone antibody should be determined by the researcher.
1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Histone antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Histone
- 别名
- Histone H2A antibody, Histone H2B 1 antibody, Histone H3 antibody, Probable histone H2B 4 antibody, Histone H2B 2 antibody, Histone antibody, his-16 antibody, his-12 antibody, his-11 antibody, his-9 antibody, his-43 antibody, his-61 antibody, his-48 antibody, his-57 antibody, his-22 antibody, his-4 antibody, his-3 antibody, his-42 antibody, his-66 antibody, his-65 antibody, Histone antibody
- 背景
- Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
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