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EpCAM 抗体

EPCAM 适用: 人 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal 323-A3 unconjugated
产品编号 ABIN3024759
发货至: 中国
  • 抗原 See all EpCAM (EPCAM) 抗体
    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
    适用
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    宿主
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    小鼠
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    单克隆
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    This EpCAM antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    无交叉反应
    大鼠
    纯化方法
    Protein G affinity chromatography
    免疫原
    MCF-7 human breast cancer cells were used as the immunogen for this EpCAM antibody.
    克隆位点
    323-A3
    亚型
    IgG1 kappa
    Top Product
    Discover our top product EPCAM Primary Antibody
  • 应用备注
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

    1. Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1 mg/mL Tris-HCl,  pH 2.0 for 15 min at RT or 10 min at 37C.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.25-0.5 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    0.2 mg/mL
    缓冲液
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
    别名
    EpCAM (EPCAM 产品)
    别名
    DIAR5 antibody, EGP-2 antibody, EGP314 antibody, EGP40 antibody, ESA antibody, HNPCC8 antibody, KS1/4 antibody, KSA antibody, M4S1 antibody, MIC18 antibody, MK-1 antibody, TACSTD1 antibody, TROP1 antibody, ECS-1 antibody, ECS1 antibody, ESA1 antibody, M17S1 antibody, EGP antibody, Ep-CAM antibody, sb:cb6 antibody, tacstd antibody, wu:fj17g02 antibody, zgc:110304 antibody, zgc:77119 antibody, tacstd1 antibody, MGC80540 antibody, EPCAM antibody, egp antibody, ksa antibody, m4s1 antibody, mk-1 antibody, cd326 antibody, egp40 antibody, mic18 antibody, trop1 antibody, ep-cam antibody, hegp-2 antibody, co17-1a antibody, ga733-2 antibody, CD326 antibody, Egp314 antibody, EpCAM1 antibody, GA733-2 antibody, Ly74 antibody, Tacsd1 antibody, Tacstd1 antibody, gp40 antibody, epithelial cell adhesion molecule antibody, flotillin 2 antibody, epithelial cell adhesion molecule S homeolog antibody, EPCAM antibody, FLOT2 antibody, epcam antibody, epcam.S antibody, Epcam antibody
    背景
    Epithelial cellular adhesion molecule (EpCAM), is also identified as epithelial specific antigen (ESA) and the 40  kDa transmembrane epithelial glycoprotein EGP40. EpCAM is expressed on the baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. EpCAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
    基因ID
    4072
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