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- 抗原 See all CD8 alpha (CD8A) 抗体
- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD8 alpha antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Human recombinant protein was used as the immunogen for this CD8a antibody.
- 克隆位点
- CLDA8a-1
- 亚型
- IgG1
- Top Product
- Discover our top product CD8A Primary Antibody
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anti-CD8a Molecule (CD8A) antibody
CD8A 适用: 人 IHC, WB 宿主: 兔 Monoclonal unconjugated
anti-CD8a Molecule (CD8A) (AA 22-235) antibodyCD8A 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 8H8A8 unconjugated
anti-CD8a Molecule (CD8A) (AA 51-150) antibodyCD8A 适用: 人, 大鼠, 小鸡 FACS, WB, ELISA, IHC (fro), IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 人 IHC, WB, IP, ELISA 宿主: 兔 Monoclonal 2F2 unconjugated Recombinant Antibody
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 小鼠 FACS, IP, IHC (fro), IHC (p), ICC 宿主: 大鼠 Monoclonal 53-6-7 unconjugated
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 小鼠 FACS, IP, IHC (fro), IHC (p), ICC, Func 宿主: 大鼠 Monoclonal 53-6-7 unconjugated
anti-CD8a Molecule (CD8A) antibody (FITC)CD8A 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 53-6-7 FITC
anti-CD8a Molecule (CD8A) antibodyCD8A 适用: 人 IHC, StM, ISt 宿主: 小鼠 Monoclonal C8-468 unconjugated
anti-CD8a Molecule (CD8A) antibody (PE)CD8A 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 53-6-7 PE
anti-CD8a Molecule (CD8A) (AA 22-182) antibodyCD8A 适用: 人 FACS, IHC, WB, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 5H5D7 unconjugated
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- 应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD8a antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,Immunohistology (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- CD8a antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- 抗原
- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
- 别名
- CD8a (CD8A 产品)
- 别名
- cd8 antibody, LOC100136450 antibody, BB154331 antibody, Ly-2 antibody, Ly-35 antibody, Ly-B antibody, Lyt-2 antibody, zgc:136643 antibody, CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, RHACD8-4 antibody, CD8a molecule antibody, uncharacterized LOC100125537 antibody, CD8 alpha antibody, CD8 antigen, alpha chain antibody, T-cell surface glycoprotein CD8 alpha chain antibody, Cd8a antibody, LOC100125537 antibody, LOC100136450 antibody, CD8A antibody, cd8a antibody, LOC100126556 antibody
- 背景
- CD8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD8 and 4 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4. CD8a antibody is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral Tcell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+/CD8-, and in T-lymphoblastic lymphoma/leukemia, they are often co-expressed. It is also found in littoral cell angioma of the spleen.
- 基因ID
- 925
- 途径
- TCR Signaling
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