Bcl-2 抗体 (AA 41-54)
Quick Overview for Bcl-2 抗体 (AA 41-54) (ABIN3024194)
抗原
See all Bcl-2 (BCL2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 41-54
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无交叉反应
- 小鼠, 大鼠
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纯化方法
- Protein G purification
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免疫原
- A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG) of the human protein was used as the immunogen for both antibodies in this Bcl2 antibody cocktail.
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亚型
- IgG1 kappa
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anti-B-Cell CLL/lymphoma 2 (BCL2) (N-Term) antibody
BCL2 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 101-160) antibodyBCL2 适用: 人, 小鼠, 大鼠, 兔, Cow, 小鸡, Bacteria WB, FACS, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 2-211) antibodyBCL2 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-B-Cell CLL/lymphoma 2 (BCL2) antibodyBCL2 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 2-211) antibodyBCL2 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D1 unconjugated
anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibodyBCL2 适用: 人 WB, IHC, FACS, IF, StM, ISt 宿主: 小鼠 Monoclonal 100-D5 unconjugated
anti-B-Cell CLL/lymphoma 2 (BCL2) antibodyBCL2 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal BCL2-2210R unconjugated Recombinant Antibody
anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibodyBCL2 适用: 人 WB, FACS, IP, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal Bcl-2-100 unconjugated
anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody (PE)BCL2 适用: 人 ICFC 宿主: 小鼠 Monoclonal Bcl-2-100 PE
anti-B-Cell CLL/lymphoma 2 (BCL2) (AA 118-165) antibodyBCL2 适用: 人 WB, FACS, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Variations in protocols, secondaries and substrates may require the antibody cocktail to be titered for optimal performance.
1. FFPE staining is enhanced by boiling tissue sections in 1 mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow: 0.5-1 μg/million cells,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunoprecipitation: 0.5-1 μg/500 μg protein lysate,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Bcl2 antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))
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别名
- Bcl2
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背景
- B-cell lymphoma 2 is an oncogene that regulates apoptosis, having both inducing and inhibiting functions. In cancer cells, Bcl2 binds pro-apoptotic proteins, preventing programmed cell death from occurring. Overexpression is seen in many cancers including lung, breast, prostate and lymphomas. In most follicular lymphomas, neoplastic germinal centers express high levels of the protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, Bcl2 antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies.
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基因ID
- 596
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途径
- MAPK Pathway, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
抗原
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