DDX58 抗体 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58)

Details for Product anti-DDX58 Antibody No. ABIN3023649
抗原
  • RIG-I
  • RIGI
  • RLR-1
  • 6430573D20Rik
  • C330021E21
  • RHIV-1
  • RIG-1
  • DExD/H-box helicase 58
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
  • DEXD/H-box helicase 58
  • DDX58
  • Ddx58
适用
小鼠, 大鼠, 人
162
69
31
2
2
宿主

126
28
10
3
克隆类型
多克隆
标记
This DDX58 antibody is un-conjugated
11
5
5
3
3
3
3
3
3
2
2
2
2
2
应用范围
Immunohistochemistry (IHC), Western Blotting (WB)
116
51
43
27
26
17
13
7
4
4
3
1
选项
免疫原 A synthetic peptide of human DDX58
亚型 IgG
纯化方法 Affinity purification
Plasmids, Primers & others Plasmids, Primers & others DDX58 products on genomics-online (e.g. as negative or positive controls)
抗原
别名 DDX58 (DDX58 Antibody 摘要)
背景 Antiviral innate immunity depends on the combination of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and RNA helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes. TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including DNA, single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and glycoproteins. These antiviral proteins are found in different cell compartments, TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection. Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling. Rig-I binds both dsRNA and viral ssRNA that contains a 5-triphosphate end not seen in host RNA. Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses. The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-kappaB, TBK1/IKKepsilon, and IRF-3/IRF-7.
Synonyms: DDX58, DKFZp434J1111, DKFZp686N19181, FLJ13599, RIG-I
基因ID 23586
UniProt O95786
途径 Activation of Innate immune Response, Hepatitis C
应用备注 Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
限制 仅限研究用
状态 Liquid
缓冲液 PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid freeze / thaw cycles
储存条件 -20 °C/-80 °C
厂商提供的图像
Western Blotting (WB) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) antibody (ABIN3023649) Western blot analysis of extracts of various cell lines, using DDX58 antibody.
Western Blotting (WB) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) antibody (ABIN3023649) Western blot analysis of extracts of various cell lines, using DDX58 antibody.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) antibody (ABIN3023649) Immunohistochemistry of paraffin-embedded human prostate using DDX58 antibody.
Immunofluorescence (IF) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) antibody (ABIN3023649) Immunofluorescence analysis of HeLa cells using DDX58 antibody.
有引用在: Zhu, Ye, Ashraf, Li, Chen, Song, Cao: "Transcriptional regulation of miR-15b by c-Rel and CREB in Japanese encephalitis virus infection." in: Scientific reports, Vol. 6, pp. 22581, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Cheng, Liao, Zhou, Peng, Chen, Yuan: "Amplified RLR signaling activation through an interferon-stimulated gene-endoplasmic reticulum stress-mitochondrial calcium uniporter protein loop." in: Scientific reports, Vol. 6, pp. 20158, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

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