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Histone 3 抗体 (H3K27me)

Cited in 14+ publications. The 兔多克隆 anti-Histone 3 antibody (ABIN3023269) specifically detects Histone 3 in WB, IHC, IF, ChIP, IP 和 ChIP-seq. The antibody is reactive with 人 samples.

Immunohistochemistry of paraffin-embedded human breast cancer using TriMethyl-Histone H3-K27 antibody (ABIN3023268, ABIN3023269, ABIN3023270, ABIN1513002 and ABIN6219521) at dilution of 1:100 (40x lens). (Histone 3 抗体 (H3K27me))

IHC

Immunohistochemistry of paraffin-embedded human rectal cancer tissue using H3K27me3 antibody at dilution of 1:200 (x400 lens). (Histone 3 抗体 (H3K27me))

IHC

Immunohistochemistry (IHC) image for anti-Histone H3 (H3) (H3K27me) antibody (ABIN3023269) (Histone 3 抗体 (H3K27me))

IHC

ChIP

Immunofluorescence analysis of 293T cell using H3K27me3 antibody. (Histone 3 抗体 (H3K27me))

Dot Blot (DB) image for anti-Histone H3 (H3) (H3K27me) antibody (ABIN3023269) (Histone 3 抗体 (H3K27me))

Immunohistochemistry of paraffin-embedded rat brain tissue using H3K27me3 antibody at dilution of 1:200 (x400 lens). (Histone 3 抗体 (H3K27me))

IHC

Immunohistochemistry of paraffin-embedded human thyroid cancer tissue using H3K27me3 antibody at dilution of 1:200 (x400 lens). (Histone 3 抗体 (H3K27me))

IHC

NNMT regulates intracellular methylation potential and CD44 transcription by inhibiting histone H3K27 methylation. (Histone 3 抗体 (H3K27me))

Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody (ABIN3023268, ABIN3023269, ABIN3023270, ABIN1513002 and ABIN6219521) at 1:1000 dilution. (Histone 3 抗体 (H3K27me))

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H3-K27 antibody (ABIN3023268, ABIN3023269, ABIN3023270, ABIN1513002 and ABIN6219521). (Histone 3 抗体 (H3K27me))

Immunohistochemistry of paraffin-embedded rat ovary using TriMethyl-Histone H3-K27 antibody (ABIN3023268, ABIN3023269, ABIN3023270, ABIN1513002 and ABIN6219521) at dilution of 1:100 (40x lens). (Histone 3 抗体 (H3K27me))

IHC

Chromatin immunoprecipitation analysis of extracts of HeLa, cells, using TriMethyl-Histone H3-K27 Rabbit pAb antibody (ABIN3023268, ABIN3023269, ABIN3023270, ABIN1513002 and ABIN6219521) and rabbit IgG. (Histone 3 抗体 (H3K27me))

ChIP

Immunofluorescence analysis of PC-12 cells using TriMethyl-Histone H3-K27 Rabbit pAb (ABIN3023268, ABIN3023269, ABIN3023270, ABIN1513002 and ABIN6219521) at dilution of 1:25 (40x lens). (Histone 3 抗体 (H3K27me))

Immunohistochemistry of paraffin-embedded mouse brain using TriMethyl-Histone H3-K27 antibody (ABIN3023268, ABIN3023269, ABIN3023270, ABIN1513002 and ABIN6219521) at dilution of 1:100 (40x lens). (Histone 3 抗体 (H3K27me))

IHC

17 images

(14 references)

产品编号 ABIN3023269

发货至: 中国

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Our Local Distributor

中国
北京 101111

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Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Histone 3 抗体 (H3K27me) (ABIN3023269)

抗原

See all Histone 3 (H3) 抗体

Histone 3 (H3) (Histone H3 (H3))

适用

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人

宿主

兔

克隆类型

多克隆

标记

This Histone 3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq)

Discover our top product H3 Primary Antibody

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H3K27me

交叉反应

人, 小鼠, 大鼠

产品特性

Methylated Antibodies

纯化方法

Affinity purification

免疫原

A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3

亚型

IgG
anti-Histone H3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (C-Term) antibody
H3 适用: 人 WB, IHC, ChIP, IP 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9me2) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K79me) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

应用备注

WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200,IP,1:50 - 1:200,ChIP,1:20 - 1:100,ChIP-seq,1:20 - 1:100

限制

仅限研究用
状态

Liquid

缓冲液

PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze / thaw cycles

储存条件

-20 °C

储存方法

Store at -20°C. Avoid freeze / thaw cycles.
Wang, Wang, Lin, Ye, Li, Tu, Shen, Li, Yang, Zhang: "Evolutionary dynamics of 3D genome architecture following polyploidization in cotton." in: Nature plants, Vol. 4, Issue 2, pp. 90-97, (2018) (PubMed).

Cheng, Tan, Lu, Liu, Li, Yuan, Zhao, Zhou: "WOX11 recruits a histone H3K27me3 demethylase to promote gene expression during shoot development in rice." in: Nucleic acids research, Vol. 46, Issue 5, pp. 2356-2369, (2018) (PubMed).

Wu, Tian, Zhang, Tong, Huang, Li, Zhao, Tang, Yuan, Wang, Fang, Gao, Hu, Li, Qin, Yao, Chen, Chen, Zhang, Liu, Sun, Chen, Wong, Ge, Chen, Ji: "In vivo CRISPR screening unveils histone demethylase UTX as an important epigenetic regulator in lung tumorigenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, Issue 17, pp. E3978-E3986, (2018) (PubMed).

Dumasia, Kumar, Deshpande, Balasinor: "Estrogen, through estrogen receptor 1, regulates histone modifications and chromatin remodeling during spermatogenesis in adult rats." in: Epigenetics, Vol. 12, Issue 11, pp. 953-963, (2018) (PubMed).

Yu, Liu, Liu, Wang, Liu, Miao, Du, Yang: "Ascorbic acid induces global epigenetic reprogramming to promote meiotic maturation and developmental competence of porcine oocytes." in: Scientific reports, Vol. 8, Issue 1, pp. 6132, (2018) (PubMed).

Song, Li, Wang, Du, Yang: "DMBA acts on cumulus cells to desynchronize nuclear and cytoplasmic maturation of pig oocytes." in: Scientific reports, Vol. 7, Issue 1, pp. 1687, (2018) (PubMed).

Tang, Guo, Zhu, Huang, Liu, Cai, Yu, Wang: "Metformin inhibits ovarian cancer via decreasing H3K27 trimethylation." in: International journal of oncology, Vol. 52, Issue 6, pp. 1899-1911, (2018) (PubMed).

Cao, Liu, Yue, Liu, Pei, Gu, Wang, Jia: "Iron chelation inhibits cancer cell growth and modulates global histone methylation status in colorectal cancer." in: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, Vol. 31, Issue 5, pp. 797-805, (2018) (PubMed).

Wang, Tu, Lin, Lin, Wang, Yang, Ye, Shen, Li, Zhang, Zhou, Nie, Li, Guo, Ma, Huang, Jin, Zhu, Yang, Min, Yuan, Zhang, Lindsey, Zhang: "Asymmetric subgenome selection and cis-regulatory divergence during cotton domestication." in: Nature genetics, Vol. 49, Issue 4, pp. 579-587, (2017) (PubMed).

Zhou, Liu, Zhou, Zhou, Zhao, Li, Zhou: "Cooperation between the H3K27me3 Chromatin Mark and Non-CG Methylation in Epigenetic Regulation." in: Plant physiology, Vol. 172, Issue 2, pp. 1131-1141, (2017) (PubMed).

Kumar, Dumasia, Deshpande, Balasinor: "Direct regulation of genes involved in sperm release by estrogen and androgen through their receptors and coregulators." in: The Journal of steroid biochemistry and molecular biology, Vol. 171, pp. 66-74, (2017) (PubMed).

Su, Li, Lei, Wang, Zhao, Cai, Wang, Li, Wu: "The EZH1-SUZ12 complex positively regulates the transcription of NF-κB target genes through interaction with UXT." in: Journal of cell science, Vol. 129, Issue 12, pp. 2343-53, (2017) (PubMed).

Yi, Guo, Guo, Sun, Yang, Wang, Li, Xie, Cai, Wang: "EZH2-mediated epigenetic silencing of TIMP2 promotes ovarian cancer migration and invasion." in: Scientific reports, Vol. 7, Issue 1, pp. 3568, (2017) (PubMed).

Liu, Zhou, Wang, Ye, Zhao, Xu, Zhou, Tan, Cheng, Zhou: "Regulation of histone methylation and reprogramming of gene expression in the rice inflorescence meristem." in: The Plant cell, Vol. 27, Issue 5, pp. 1428-44, (2016) (PubMed).
抗原

Histone 3 (H3) (Histone H3 (H3))

别名

Histone H3

背景

Actin is a key regulator of RNA polymerase (Pol) II-dependent transcription. Positive transcription elongation factor b (P-TEFb), a Cdk9/cyclin T1 heterodimer, has been reported to play a critical role in transcription elongation. However, the relationship between actin and P-TEFb is still not clear. In this study, actin was found to interact with Cdk9, a catalytic subunit of P-TEFb, in elongation complexes. Using immunofluorescence and immunoprecipitation assays, Cdk9 was found to bind to G-actin through the conserved Thr-186 in the T-loop. Overexpression and in vitro kinase assays showed that G-actin promotes P-TEFb-dependent phosphorylation of the Pol II C-terminal domain. An in vitro transcription experiment revealed that the interaction between G-actin and Cdk9 stimulated Pol II transcription elongation. ChIP and immobilized template assays indicated that actin recruited Cdk9 to a transcriptional template in vivo and in vitro. Using cytokine IL-6-inducible p21 gene expression system, we revealed that actin recruited Cdk9 to endogenous gene. Moreover, overexpression of actin and Cdk9 increased histone H3 acetylation and acetylized histone H3 binding to a transcriptional template through the interaction with histone acetyltransferase, p300. Taken together, our results suggested that actin participates in transcription elongation by recruiting Cdk9 for phosphorylation of the Pol II C-terminal domain, and the actin-Cdk9 interaction promotes chromatin remodeling.,H3.4,H3/g,H3FT,H3t,HIST3H3,Histone H3,HIST1H3A,Signal Transduction,MAPK-Erk Signaling Pathway,MAPK-P38 Signaling Pathway,Epigenetics & Nuclear Signaling,Epigenetic Modifications,Methylation,Histone H3

分子量

15 kDa

基因ID

8290

UniProt

Q16695