GTPase NRas 抗体 (Internal Region)
Quick Overview for GTPase NRas 抗体 (Internal Region) (ABIN302138)
抗原
See all GTPase NRas (NRAS) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- NRAS
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序列
- RMKKLNSSDD GTQ
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.
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亚型
- IgG
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anti-GTPase NRas (NRAS) (AA 1-189) antibody
NRAS 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-GTPase NRas (NRAS) (AA 72-101) antibodyNRAS 适用: 人 WB, ELISA, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-GTPase NRas (NRAS) (AA 147-179) antibodyNRAS 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-GTPase NRas (NRAS) (AA 101-189) antibodyNRAS 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-GTPase NRas (NRAS) (AA 91-140) antibodyNRAS 适用: 人, 大鼠, 小鼠, 兔, Cow, 豚鼠, 马, 斑马鱼, Bat, 猴, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-GTPase NRas (NRAS) antibodyNRAS 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-GTPase NRas (NRAS) (AA 72-101), (N-Term) antibodyNRAS 适用: 人, 大鼠, 小鼠 WB, FACS, IF 宿主: 兔 Polyclonal RB14459 unconjugated
anti-GTPase NRas (NRAS) (AA 1-186) antibodyNRAS 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-GTPase NRas (NRAS) antibodyNRAS 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB56819 unconjugated
anti-GTPase NRas (NRAS) (AA 90-189) antibodyNRAS 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A3 unconjugated
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应用备注
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Western Blot: Approx. 21-22 kDa band observed in lysates of cell line MCF7 (calculated MW of 21.2 kDa according to NP_002515.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:64000.
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说明
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Immunofluorescence: A customer obtained weak cytoplasmic staining in MCF7 cells at 10μg/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- GTPase NRas (NRAS)
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别名
- NRAS
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背景
- NRAS , neuroblastoma RAS viral (v-ras) oncogene homolog , N-ras , NRAS1, N-ras protein part 4 , v-ras neuroblastoma RAS viral oncogene homolog
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基因ID
- 4893, 18176, 24605
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NCBI登录号
- NP_002515
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途径
- p53 Pathway, MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
抗原
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