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FZD2 抗体 (Internal Region)

This anti-FZD2 antibody is a 山羊 多克隆 antibody detecting FZD2 in WB, ELISA, IF 和 IHC. Suitable for 人. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN302136
发货至: 中国

Quick Overview for FZD2 抗体 (Internal Region) (ABIN302136)

抗原

See all FZD2 抗体
FZD2 (Frizzled Family Receptor 2 (FZD2))

适用

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宿主

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山羊

克隆类型

  • 40
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多克隆

标记

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This FZD2 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

质量等级

Verified
  • 抗原表位

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    Internal Region

    原理

    Frizzled 2

    序列

    RYATLEHPFH CPR

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration: 2-5 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.

    Western Blot: Approx 60-65 kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24 kDa was also observed in some lysates which was successfully blocked by incubation with

    Peptide ELISA: antibody detection limit dilution 1:64000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Hirano, Yonezawa, Yunoue, Habu, Uchida, Yoshioka, Kishida, Kishida, Oyoshi, Fujio, Sugata, Yamahata, Hanaya, Arita: "Immunoreactivity of Wnt5a, Fzd2, Fzd6, and Ryk in glioblastoma: evaluative methodology for DAB chromogenic immunostaining." in: Brain tumor pathology, Vol. 31, Issue 2, pp. 85-93, (2014) (PubMed).

  • 抗原

    FZD2 (Frizzled Family Receptor 2 (FZD2))

    别名

    FZD2

    背景

    FZD2 , frizzled homolog 2 (Drosophila) , frizzled 2

    基因ID

    2535, 57265, 64512

    NCBI登录号

    NP_001457

    途径

    WNT signaling
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