FZD2 抗体 (Internal Region)
Quick Overview for FZD2 抗体 (Internal Region) (ABIN302136)
抗原
See all FZD2 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- Frizzled 2
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序列
- RYATLEHPFH CPR
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.
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亚型
- IgG
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应用备注
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Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration: 2-5 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx 60-65 kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24 kDa was also observed in some lysates which was successfully blocked by incubation with
Peptide ELISA: antibody detection limit dilution 1:64000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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Immunoreactivity of Wnt5a, Fzd2, Fzd6, and Ryk in glioblastoma: evaluative methodology for DAB chromogenic immunostaining." in: Brain tumor pathology, Vol. 31, Issue 2, pp. 85-93, (2014) (PubMed).
: "
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Immunoreactivity of Wnt5a, Fzd2, Fzd6, and Ryk in glioblastoma: evaluative methodology for DAB chromogenic immunostaining." in: Brain tumor pathology, Vol. 31, Issue 2, pp. 85-93, (2014) (PubMed).
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- FZD2 (Frizzled Family Receptor 2 (FZD2))
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别名
- FZD2
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背景
- FZD2 , frizzled homolog 2 (Drosophila) , frizzled 2
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基因ID
- 2535, 57265, 64512
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NCBI登录号
- NP_001457
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途径
- WNT signaling
抗原
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