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RAF1 抗体 (AA 657-668)

Name: RAF1 抗体 (AA 657-668)
SKU: ABIN295906
Availability: OutOfStock

RAF1 适用: 人, 小鼠, 大鼠, Cow, 猴, 兔, 犬, 马, Bat, Hamster WB, IP 宿主: 绵羊 Polyclonal unconjugated

产品编号 ABIN295906

发货至: 中国

Datasheet as PDF

抗原 See all RAF1 抗体

RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
抗原表位
48

43

35

34

32

27

24

23

22

22

19

18

15

13

13

9

8

8

7

6

6

6

6

5

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1
AA 657-668
适用
445

230

158

13

12

12

11

8

4

4

4

2

2

2

1

1
人, 小鼠, 大鼠, Cow, 猴, 兔, 犬, 马, Bat, Hamster
宿主
477

15

2

1
绵羊
克隆类型
453

42
多克隆
标记
200

37

28

27

20

20

20

20

20

20

17

16

10

10

10

10

10
This RAF1 antibody is un-conjugated
应用范围
295

217

105

104

89

75

49

31

30

24

16

10

10

3

2

2

1
Western Blotting (WB), Immunoprecipitation (IP)

特异性

Recognizes Raf-1, Mr ~70kD. Species cross-reactivity: Human. Predicted to cross-react with mouse and rat based on sequence homology.

预测反应

Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%).

纯化方法

Protein A purified

免疫原

12 residue synthetic C terminal peptide (CTLTTSPRLPVF) corresponding to residues 637-648 of human Raf-1. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit (100%).

Type of Immunogen: Synthetic peptide

亚型

IgG

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Discover our top product RAF1 Primary Antibody
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 31-130) antibody
RAF1 适用: 人 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 190-350) antibody
RAF1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 1-130) antibody
RAF1 适用: 人 WB, ELISA, RNAi, PLA 宿主: 小鼠 Monoclonal 1H4 unconjugated

anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody
RAF1 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4G4 unconjugated

anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer621) antibody
RAF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer259) antibody
RAF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 1-648) antibody
RAF1 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody
RAF1 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer338) antibody
RAF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer621) antibody
RAF1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

应用备注

Approved: IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 2 μg/mL detects Raf-1 in RIPA lysates from 3T3/A31 cell lysates. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Raf-1 (2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Included Positive Antigen Control: 3T3/A31 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. MAPK Activation and MBP Phosphorylation: 4 μg immunoprecipitates Raf-1. The immunocomplex was then used in a coupled phosphorylation assay to activate MEK1, which in turn activates MAP Kinase 2 that phosphorylates myelin basic protein in vitro.

说明

Target Species of Antibody: Human

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeat freeze-thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
抗原

RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

别名

RAF1 / RAF (RAF1 产品)

别名

CRAF antibody, NS5 antibody, Raf-1 antibody, c-Raf antibody, c-raf antibody, craf antibody, raf1 antibody, RAF1 antibody, 6430402F14Rik antibody, AA990557 antibody, BB129353 antibody, Craf1 antibody, D830050J10Rik antibody, v-Raf antibody, raf antibody, cRaf antibody, Raf-1 proto-oncogene, serine/threonine kinase antibody, Raf-1 proto-oncogene, serine/threonine kinase a antibody, v-raf-leukemia viral oncogene 1 antibody, Raf-1 proto-oncogene, serine/threonine kinase S homeolog antibody, RAF1 antibody, Raf1 antibody, raf1a antibody, raf1 antibody, raf1.S antibody

背景

Name/Gene ID: RAF1
Subfamily: Raf
Family: Protein Kinase

Synonyms: RAF1, C-Raf, C-raf-1, CRAF, NS5, Raf-1, Proto-oncogene c-RAF, Oncogene RAF1, RAF, Raf1 kinase

基因ID

5894

UniProt

P04049

途径

MAPK Pathway, RTK signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling