HLA-ABC 抗体
Quick Overview for HLA-ABC 抗体 (ABIN294012)
抗原
See all HLA-ABC 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Reacts with all human classical MHC Class I molecules in native cell-surface forms (e. g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. The antibody MEM-147 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A. Reactivity is very similar to the classical antibody W6/32.
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纯化方法
- Protein A purified
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免疫原
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PHA-activated peripheral blood lymphocytes.
Type of Immunogen: Cells -
亚型
- IgG1
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应用备注
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Approved: Flo, IP, WB
Usage: Flow Cytometry: Recommended dilution - 1 μg/mL. Western Blotting: Application note - Non-reducing conditions.
Not recommended for: IHC-P -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, 15 mM sodium azide, approx, pH 7.4.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- HLA-ABC
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别名
- HLA-A/B/C
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途径
- TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
抗原
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