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HES4 抗体 (AA 94-221)

HES4 适用: 人 WB, ELISA, ICC 宿主: 绵羊 Polyclonal unconjugated
产品编号 ABIN291868
发货至: 中国
  • 抗原 See all HES4 抗体
    HES4 (Hairy and Enhancer of Split 4 (HES4))
    抗原表位
    • 2
    • 2
    • 1
    • 1
    • 1
    AA 94-221
    适用
    • 9
    • 2
    • 1
    • 1
    • 1
    • 1
    宿主
    • 5
    • 2
    • 1
    • 1
    绵羊
    克隆类型
    • 9
    多克隆
    标记
    • 8
    • 1
    This HES4 antibody is un-conjugated
    应用范围
    • 8
    • 4
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
    特异性
    Selected for its ability to recognize human HES-4 in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 1 % cross-reactivity with rhHES-1, rhHES-2, rhHES-5, and rhHES-7.
    纯化方法
    Affinity purified
    免疫原
    E. coli-derived rhHES-4 (aa94-221).
    亚型
    IgG
    Top Product
    Discover our top product HES4 Primary Antibody
  • 应用备注
    Approved: ELISA, ICC, WB

    Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect HES-4 in HEK293 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 0.2 ng/well.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    deionized water
    浓度
    Lot specific
    缓冲液
    Lyophilized.
  • 抗原
    HES4 (Hairy and Enhancer of Split 4 (HES4))
    别名
    HES4 (HES4 产品)
    别名
    bHLHb42 antibody, XHairy2a antibody, Xhairy2 antibody, Xhairy2b antibody, hairy2 antibody, hairy2a antibody, hairy2b antibody, hes1 antibody, hes4a antibody, hes4b antibody, Xhairy2a antibody, hes family bHLH transcription factor 4 antibody, hes family bHLH transcription factor 4 L homeolog antibody, hes family bHLH transcription factor 4 S homeolog antibody, HES4 antibody, hes4 antibody, hes4.L antibody, hes4.S antibody
    背景
    Name/Gene ID: HES4

    Synonyms: HES4, BHLHb42, Transcription factor HES-4, BHLH factor Hes4, Hairy and enhancer of split 4, HHES4
    基因ID
    57801
    UniProt
    Q9HCC6
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