Macrophage Surface Antigen 抗体
适用: 人
IHC
宿主: 小鼠
Monoclonal
AM-3K
unconjugated
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- 抗原
- Macrophage Surface Antigen
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Immunohistochemistry (IHC)
- 纯化方法
- The splenic lymphocytes from BALB/c mouse, immunized with human alveolarmacrophages, were fused to myeloma NS-1 cells. The screening of thehybridoma cells was performed on cryostat sections of human lung. The cellline (AM-3K) with positive reaction was grown in ascitic fluid of BALB/cmouse, from which the antibody was purified by Protein G affinity chromatography.
- 免疫原
- human alveolar macrophages
- 克隆位点
- AM-3K
- 亚型
- IgG1
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- 应用备注
- Working dilution for immunohistochemistry: 10ug/ml, on frozen sections and paraffin sections. Antigen retrieval (microwave 10min, 0.01M citrate buffer, pH2.0) recommended.
- 限制
- 仅限研究用
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- 浓度
- 0.25 mg/ml
- 缓冲液
- PBS (containing 2% Block Ace as a stabilizer, 0.1% Proclin as a bacteriostat)
- 储存液
- ProClin
- 储存条件
- -20 °C
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- 抗原
- Macrophage Surface Antigen
- 背景
- This anti-macrophage monoclonal antibody, AM-3K, was produced by using human alveolarmacrophages as immunogen. AM-3K reacts intensely with most of macrophages in lymphoreticular organsand in many other organs and tissues. AM-3K also reacts with the macrophages in many pathologicalconditions. However, this antibody does not react with dendritic cell population, such as epidermal Langerhans cells, interdigitating cells in the paracortex of lymph nodes, nor follicular dendritic cells.Lymphocytes, granulocytes and freshly isolated blood monocytes are also negative. Reaction products for AM-3K were found on the cytoplasmic membrane of macrophages by immunoelectron microscopy. In both cryostat sections and formalin-fixed paraffin sections, this antibody recognizes the antigenpresenting on the cell surface membrane of tissue macrophages, but not monocytes or dendritic cells. The molecular weights of the antigen recognized by AM-3K are 120 and 70 kDa.
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