This antibody will recognize wild type Rev protein produced from both in vivo (expression in COS, CV-1, HeLa cell lines) and in vitro (E. coli bacterial expressionsystem and translation products). Very low crossreactivity with other E. coli proteinson western blot. Crossreactivity with other GST fusion proteins will occur unless preabsorbed against GST.
交叉反应
小鼠, 大鼠
交叉反应 (详细)
This antibody reacts with residues 125-142 EETAKKVRRAIEQLAAMD of the human Survivin protein. Cross reacts with mouse and rat Survivin (88% sequenceidentity).
纯化方法
Affinity chromatography purified
免疫原
Survivin antibody was raised in rabbit using AA 125-142 EETAKKVRRAIEQLAAMD of the human survivin protein as the immunogen.
Survivin is a human gene encoding a structurally unique IAP apoptosis inhibitor. Present during fetal development, survivin is undetectable in terminally differentiated adult tissues. However, survivin becomes prominently expressed in transformed cell lines and in all the most common human cancers of lung, colon, pancreas, prostate and breast, in vivo.