ATP6V1H 抗体 (Center)
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- 抗原 See all ATP6V1H 抗体
- ATP6V1H (ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H))
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抗原表位
- Center
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ATP6V1H antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 交叉反应
- 小鸡, 猪, 非洲爪蟾, 斑马鱼, 牛, Xenopus tropicalis
- 交叉反应 （详细）
- Chicken (96 %), Pig (99 %), Xenopus laevis (94 %), Zebrafish (91 %), Bovine (98 %), Xenopus tropicalis (94 %)
- 产品特性
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Rabbit Polyclonal antibody to ATP6V1H (ATPase, H+ transporting, lysosomal 50/57 kDa, V1 subunit H)
ATP6V1H antibody [N3C3] - 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Recombinant protein encompassing a sequence within the center region of human ATP6V1H. The exact sequence is proprietary.
- 亚型
- IgG
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anti-ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H) (AA 1-483) antibody
ATP6V1H 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H) (Internal Region) antibodyATP6V1H 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H) antibodyATP6V1H 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H) (Internal Region) antibodyATP6V1H 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H) (AA 1-483) antibodyATP6V1H 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
- Suggested dilution Reference ICC/IF Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IFAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
- 说明
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Positive Control: 293T , A431 , H1299 , HeLa , Raji , mouse brain
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.56 mg/mL
- 缓冲液
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- 抗原
- ATP6V1H (ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H))
- 别名
- ATP6V1H (ATP6V1H 产品)
- 别名
- MSTP042 antibody, NBP1 antibody, SFD antibody, SFDalpha antibody, SFDbeta antibody, VMA13 antibody, V-ATPASE antibody, 0710001F19Rik antibody, AU022349 antibody, CGI-11 antibody, chunp6890 antibody, ATPase H+ transporting V1 subunit H antibody, ATPase, H+ transporting, lysosomal V1 subunit H antibody, ATP6V1H antibody, Atp6v1h antibody, atp6v1h antibody
- 背景
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This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the regulatory H subunit of the V1 domain which is required for catalysis of ATP but not the assembly of V-ATPase. Three alternatively spliced transcript variants encode two isoforms of the H subunit.
- 分子量
- 56 kDa
- 基因ID
- 51606
- 途径
- Transition Metal Ion Homeostasis, Proton Transport
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