Proteasome 20S alpha 1 (Center) 抗体
适用: 人, 小鼠, 大鼠
ICC, IF, IHC (p), WB
宿主: 兔
Polyclonal
unconjugated
-
- 抗原
- Proteasome 20S alpha 1
- 抗原表位
- Center
- 适用
- 人, 小鼠, 大鼠
- 宿主
- 兔
- 克隆类型
- 多克隆
- 应用范围
- Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
- 交叉反应
- 猕猴, 牛
- 交叉反应 （详细）
- Rhesus Monkey (100 %), Bovine (100 %)
- 产品特性
-
Rabbit Polyclonal antibody to Proteasome 20S alpha 1 (proteasome (prosome, macropain) subunit, alpha type, 1)
Proteasome 20S alpha 1 antibody [N1C3] - 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 1. The exact sequence is proprietary.
- 亚型
- IgG
-
- 应用备注
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
- 说明
-
Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
-
- 抗原
- Proteasome 20S alpha 1
- 背景
-
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Cellular Localization: Cytoplasm , Nucleus - 分子量
- 30 kDa
- 基因ID
- 5682
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