我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

GRP78 抗体 (Internal Region)

Name: GRP78 抗体 (Internal Region)
Brand: antibodies-online
SKU: ABIN2855429
Availability: OutOfStock

HSPA5 适用: 人, 大鼠, 小鼠 WB, IF, ICC, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated

WB Image Grp78 antibody detects Grp78 protein by western blot analysis. (GRP78 抗体 (Internal Region))

ICC/IF Image Grp78 antibody [N2C1], Internal detects Grp78 protein at endoplasmic reticulum by immunofluorescent analysis. (GRP78 抗体 (Internal Region))

IHC-P Image Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in mouse brain by immunohistochemical analysis. (GRP78 抗体 (Internal Region))

IHC

IHC-P Image Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on rat hind brain by immunohistochemical analysis. (GRP78 抗体 (Internal Region))

IHC

IHC-P Image Grp78 antibody [N2C1], Internal detects Grp78 protein at cytosol on mouse intestine by immunohistochemical analysis. (GRP78 抗体 (Internal Region))

IHC

IHC-P Image Grp78 antibody [N2C1], Internal detects Grp78 protein at cytoplasm in rat prostate by immunohistochemical analysis. (GRP78 抗体 (Internal Region))

IHC

WB Image Various whole cell extracts (30 μg) were separated by 7. (GRP78 抗体 (Internal Region))

IP Image Immunoprecipitation of Grp78 protein from HepG2 whole cell extracts using 5 μg of Grp78 antibody [N2C1], Internal, Western blot analysis was performed using Grp78 antibody [N2C1], Internal, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (GRP78 抗体 (Internal Region))

9 images

(2 references)

产品编号 ABIN2855429

发货至: 中国

Datasheet as PDF

抗原 See all GRP78 (HSPA5) 抗体

GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
抗原表位
26

16

13

8

6

6

6

6

5

5

4

4

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region
适用
184

122

105

39

38

36

34

32

31

20

15

6

5

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
人, 大鼠, 小鼠
宿主
130

89

9

3

1

1
兔
Compatible Secondaries
克隆类型
133

101
多克隆
标记
99

21

17

14

13

12

9

8

8

8

3

2

2

2

2

2

2

2

2

2

2

2
This GRP78 antibody is un-conjugated
应用范围
219

104

86

85

75

34

31

27

26

20

13

7

6

3

3

2

1
Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

交叉反应

小鸡, 牛, 猕猴

交叉反应（详细）

Mouse (100 %), Rat (100 %), Chicken (100 %), Rhesus Monkey (100 %), Bovine (100 %)

产品特性

Rabbit polyclonal antibody to Grp78 (heat shock 70 kDa protein 5 (glucose-regulated protein, 78 kDa))
Grp78 antibody [N2C1], Internal

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary.

亚型

IgG

Top Product

Discover our top product HSPA5 Primary Antibody
anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 550-650) antibody
HSPA5 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 251-360) antibody
HSPA5 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 550-650) antibody
HSPA5 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (HRP)
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal HRP

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (FITC)
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal FITC

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (Biotin)
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Biotin

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (PE)
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal PE

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (APC)
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal APC

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (PerCP)
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal PerCP

应用备注

Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*

说明

Positive Control: 293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2

限制

仅限研究用
状态

Liquid

浓度

0.72 mg/mL

缓冲液

1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Cai, Han, Hu, Zhao, He, Chen, Sun, Yuan, Li, Yang, Kong, Kong: "Metformin attenuates the D‑galactose‑induced aging process via the UPR through the AMPK/ERK1/2 signaling pathways." in: International journal of molecular medicine, Vol. 45, Issue 3, pp. 715-730, (2020) (PubMed).

Chen, Zhao, Cai, Sun, Hu, Huang, Kong, Kong: "The role of sodium hydrosulfide in attenuating the aging process via PI3K/AKT and CaMKKβ/AMPK pathways." in: Redox biology, Vol. 12, pp. 987-1003, (2017) (PubMed).
抗原

GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

别名

Grp78 (HSPA5 产品)

别名

GRP78 antibody, BiP antibody, GRP-78 antibody, grp78 antibody, hspa5a antibody, BIP antibody, MIF2 antibody, AL022860 antibody, AU019543 antibody, Bip antibody, D2Wsu141e antibody, D2Wsu17e antibody, Grp78 antibody, Hsce70 antibody, SEZ-7 antibody, Sez7 antibody, baffled antibody, mBiP antibody, cb865 antibody, fb60h09 antibody, fi36d04 antibody, wu:fb60h09 antibody, wu:fi36d04 antibody, zgc:55994 antibody, zgc:77606 antibody, 78 kDa glucose-regulated protein antibody, heat shock protein family A (Hsp70) member 5 antibody, BiP/GRP78 antibody, glucose-regulated protein 78 antibody, putative glucose-regulated protein 78 antibody, Hsp70 family ATPase KAR2 antibody, heat shock protein family A (Hsp70) member 5 S homeolog antibody, heat shock 70 kDa protein 5a antibody, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) antibody, heat shock protein 5 antibody, heat shock protein family A member 5 antibody, CpipJ_CPIJ003550 antibody, HSPA5 antibody, grp78 antibody, LOC100533358 antibody, BiP/grp78 antibody, Tc00.1047053506585.40 antibody, Tb11.02.5450 antibody, Tb11.02.5500 antibody, LMJF_28_1200 antibody, KAR2 antibody, LOC100135840 antibody, hspa5.S antibody, hspa5 antibody, Hspa5 antibody

背景

When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]

Cellular Localization: Endoplasmic reticulum lumen , Melanosome

分子量

72 kDa

基因ID

3309

途径

Thyroid Hormone Synthesis, ER-Nucleus Signaling