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KAP1 抗体

This anti-KAP1 antibody is a 兔 多克隆 antibody detecting KAP1 in WB, IF, IP, ICC, IHC (p) 和 ChIP. Suitable for 人. This Primary Antibody has been cited in 4+ publications.
产品编号 ABIN2855331
发货至: 中国

Quick Overview for KAP1 抗体 (ABIN2855331)

抗原

See all KAP1 (TRIM28) 抗体
KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

适用

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宿主

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克隆类型

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多克隆

标记

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This KAP1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)
  • 交叉反应

    人, 小鼠

    产品特性

    Rabbit Polyclonal antibody to KAP1 (tripartite motif containing 28)
    KAP1 antibody [N3C2], Internal

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: NIH-3T3

    Validation: Orthogonal

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.48 mg/mL

    缓冲液

    1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Liu, Li, Liu, Li, Wei, Liu, Lv: "Expression and Significance of TRIM 28 in Squamous Carcinoma of Esophagus." in: Pathology oncology research : POR, Vol. 25, Issue 4, pp. 1645-1652, (2020) (PubMed).

    Hung, Johnson, Chen, Byrum, Bredemeyer, Yewdell, Johnson, Lee, Deivasigamani, Hindi, Amatya, Gross, Paull, Pisapia, Chaudhuri, Petrini, Mosammaparast, Amarasinghe, Zha, Tyler, Sleckman: "MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining." in: Molecular cell, Vol. 71, Issue 2, pp. 332-342.e8, (2019) (PubMed).

    Wang, Wolgemuth: "BET Protein BRDT Complexes With HDAC1, PRMT5, and TRIM28 and Functions in Transcriptional Repression During Spermatogenesis." in: Journal of cellular biochemistry, Vol. 117, Issue 6, pp. 1429-38, (2016) (PubMed).

    Aslanian, Yates, Hunter: "Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells." in: DNA repair, Vol. 15, pp. 29-38, (2014) (PubMed).

  • 抗原

    KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

    别名

    tripartite motif containing 28

    背景

    The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

    Cellular Localization: Nucleus (By similarity)

    分子量

    89 kDa

    基因ID

    10155

    UniProt

    Q13263

    途径

    Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus
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