SLC32A1 抗体 (N-Term)
(1 validation)-
- 抗原 See all SLC32A1 抗体
- SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))
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抗原表位
- N-Term
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SLC32A1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Multiplex Immunohistochemistry (mIHC)
- 产品特性
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Rabbit Polyclonal antibody to VGAT (solute carrier family 32 (GABA vesicular transporter), member 1)
VGAT antibody [N1N2], N-term - 纯化方法
- Purified by antigen-affinity chromatography.
- 免疫原
- Recombinant protein encompassing a sequence within the N-terminus region of human VGAT. The exact sequence is proprietary.
- 亚型
- IgG
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anti-Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1) (AA 14-118) antibody
SLC32A1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1) (N-Term) antibodySLC32A1 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1) antibodySLC32A1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1) (AA 106-120), (Cytosolic), (N-Term) antibodySLC32A1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1) (AA 106-120), (Cytosolic), (N-Term) antibodySLC32A1 适用: 人, 小鼠, 大鼠 WB 宿主: 豚鼠 Polyclonal unconjugated
anti-Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1) (AA 351-450) antibodySLC32A1 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1) antibodySLC32A1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1) (N-Term) antibody (PE)SLC32A1 适用: 小鼠 WB, ELISA, IHC 宿主: 小鼠 Monoclonal D9 PE
anti-Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1) (N-Term) antibody (PerCP)SLC32A1 适用: 小鼠 WB, ELISA, IHC 宿主: 小鼠 Monoclonal D9 PerCP
anti-Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1) (N-Term) antibody (Atto 488)SLC32A1 适用: 小鼠 WB, ELISA, IHC 宿主: 小鼠 Monoclonal D9 Atto 488
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- 应用备注
- Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
- 说明
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Positive Control: U87-MG , mouse brain
- 限制
- 仅限研究用
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- by
- Akoya Biosciences
- No.
- #104339
- 日期
- 2021.04.20
- 抗原
- SLC32A1
- Lot Number
- 42838
- Method validated
- Multiplex Immunohistochemistry
- Positive Control
FFPE normal human cortex
- Negative Control
Unlabeled control (FFPE normal human cortex)
- Notes
Passed. The anti-SLC32A1 antibody ABIN2855225 produces punctate staining in close approximation with dendritic processes. This pattern is consistent with presynaptic staining, which is the expected site of SLC31A1 immunofluorescence.
- Primary Antibody
- ABIN2855225
- Secondary Antibody
- Full Protocol
- Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
- Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
- Conjugation of the anti-SLC32A1 antibody ABIN2855225 to an oligo barcode used to bind oligo-conjugated fluorophore AF488.
- Experimental Notes
No signal was detected in unlabeled specimens.
Specific staining of SLC32A1 was observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.
Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).
生效 #104339 (Multiplex Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![FFPE normal human cortex tissue section labeled with anti-SLC32A1 antibody ABIN2855225 (green; bound to fluorophore AF488) after EDTA antigen retrieval. MAP2 and SYN1 were labeled with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 550) and anti-SYN1 antibody ABIN5542390 (blue; bound to fluorophore AF488).]()
FFPE normal human cortex tissue section labeled with anti-SLC32A1 antibody ABIN2855225 (green; bound to fluorophore AF488) after EDTA antigen retrieval. MAP2 and SYN1 were labeled with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 550) and anti-SYN1 antibody ABIN5542390 (blue; bound to fluorophore AF488).
Full Methods -
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- 抗原
- SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))
- 别名
- VGAT (SLC32A1 产品)
- 别名
- VGAT antibody, VIAAT antibody, vGAT antibody, Ci-vGAT antibody, slc32a1 antibody, viaat antibody, MGC68938 antibody, vgat antibody, xVIAAT antibody, R75019 antibody, Viaat antibody, CG8394 antibody, CG8394.2 antibody, Dmel\\CG8394 antibody, Vgat antibody, zgc:158324 antibody, solute carrier family 32 member 1 antibody, vesicular GABA transporter antibody, solute carrier family 32 member 1 S homeolog antibody, vesicular inhibitory amino acid transporter antibody, Vesicular inhibitory amino acid transporter antibody, solute carrier family 32 (GABA vesicular transporter), member 1 antibody, Vesicular GABA Transporter antibody, SLC32A1 antibody, vgat antibody, Bm1_20950 antibody, slc32a1.S antibody, slc32a1 antibody, CpipJ_CPIJ002704 antibody, viaat antibody, Slc32a1 antibody, VGAT antibody, Tsp_04599 antibody
- 背景
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The protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II.
Cellular Localization: Cytoplasmic vesicle membrane, Multi-pass membrane protein - 分子量
- 57 kDa
- 基因ID
- 140679
- 途径
- Proton Transport
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