E2F1 抗体
Quick Overview for E2F1 抗体 (ABIN2854981)
抗原
See all E2F1 抗体适用
宿主
克隆类型
标记
应用范围
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交叉反应
- 人
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产品特性
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Rabbit Polyclonal antibody to E2F1 (E2F transcription factor 1)
E2F1 antibody [N1N3] -
纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the center region of human E2F1. The exact sequence is proprietary.
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亚型
- IgG
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anti-E2F Transcription Factor 1 (E2F1) (AA 69-223) antibody
E2F1 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 8G9 unconjugated
anti-E2F Transcription Factor 1 (E2F1) antibodyE2F1 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Monoclonal 1D12 unconjugated Recombinant Antibody
anti-E2F Transcription Factor 1 (E2F1) (AA 348-437) antibodyE2F1 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2E10 unconjugated
anti-E2F Transcription Factor 1 (E2F1) (AA 101-180) antibodyE2F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), FACS, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 1 (E2F1) (AA 133-362) antibodyE2F1 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 1 (E2F1) (AA 342-371) antibodyE2F1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 1 (E2F1) (pSer364) antibodyE2F1 适用: 人 WB, ELISA, IF (p), IF (cc), FACS, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 1 (E2F1) (pThr433) antibodyE2F1 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 1 (E2F1) (C-Term) antibodyE2F1 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-E2F Transcription Factor 1 (E2F1) antibodyE2F1 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: E2F1-transfected 293T
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- E2F1 (E2F Transcription Factor 1 (E2F1))
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别名
- E2F transcription factor 1
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背景
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The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Cellular Localization: Nucleus -
分子量
- 47 kDa
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基因ID
- 1869
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UniProt
- Q01094
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途径
- p53 Pathway, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
抗原
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