我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

PARP1 抗体

Name: PARP1 抗体
Brand: antibodies-online
SKU: ABIN2854798
Availability: OutOfStock

This anti-PARP1 antibody is a 兔多克隆 antibody detecting PARP1 in WB, IP, IF, ICC, IHC (p) 和 ChIP. Suitable for 人. This Primary Antibody has been cited in 7+ publications.

WB Image PARP1 antibody detects PARP1 protein by Western blot analysis. (PARP1 抗体)

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PARP1, antibody at 1:200 dilution. (PARP1 抗体)

ChIP

WB Image Sample (30 ug of whole cell lysate) A: 293T B: HeLa 7. (PARP1 抗体)

Expression of apoptogenic proteins in MCME-treated cancer cells. (PARP1 抗体)

Combined treatment of gefitinib and lovastatin induced apoptosis and autophagy in SSP-25 cellsThe cells (1 x 106/well) were seeded in a six-well tray and treated with a combination of gefitinib and lovastatin for 24 hours. (PARP1 抗体)

GSH inhibited the activation of Cd-induced p38 MAPK/JNK pathways and proteins involved in apoptosis signalingBEAS-2B cells were sham-exposed or dosed with 30 μM CdCl2 for 6, 12, or 24 h (A) or with 30 μM CdCl2 for 24 or 36 h (B), cells were lysed, and protein extracts were subjected to western blot analyses using antibodies against p-p38 MAPK, p38 MAPK, p-JNK, JNK, p-c-Jun, c-Jun, Bax, Cytochrome c, Caspase-9, Caspase-3, and PARP1. (PARP1 抗体)

The association of PARP-1 with CCR6DNP is sequence dependent and allele specific. (PARP1 抗体)

ChIP

CCR6 expression in PARP-1 knockdown human cells. (PARP1 抗体)

CCR6 expression in human cells treated with 3-aminobenzamide. (PARP1 抗体)

Partial characterization of DSB repair complexes by co-IP analysis. (PARP1 抗体)

CISD2 expression affects cell apoptosis. (PARP1 抗体)

17 images

(7 references)

产品编号 ABIN2854798

发货至: 中国

Quick Overview for PARP1 抗体 (ABIN2854798)

抗原

See all PARP1 抗体

PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

适用

人

宿主

兔

克隆类型

多克隆

标记

This PARP1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

质量等级

KO Validated

Discover our top product PARP1 Primary Antibody

交叉反应

人, 小鼠, 大鼠

产品特性

Rabbit Polyclonal antibody to PARP (poly (ADP-ribose) polymerase 1)
PARP antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary.

亚型

IgG
anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 81-390) antibody
PARP1 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 201-300) antibody
PARP1 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 661-881) antibody
PARP1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 1-100) antibody
PARP1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3G4 unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 670-858) antibody
PARP1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) antibody
PARP1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Monoclonal 8C7 unconjugated Recombinant Antibody

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (cleaved) antibody
PARP1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (N-Term) antibody
PARP1 适用: 人 WB, IHC, 2D-PAGE 宿主: 兔 Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (C-Term) antibody
PARP1 适用: 人 WB, 2D-PAGE 宿主: 兔 Polyclonal unconjugated

anti-Poly (ADP-Ribose) Polymerase 1 (PARP1) (AA 183-214) antibody
PARP1 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: HCT116 (30uM cisplatin treatment for 24hr) , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2
Validation: Comparison, KO/KD, Orthogonal, Overexpression

限制

仅限研究用
状态

Liquid

浓度

0.3 mg/mL

缓冲液

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Takino, Sato, Nagamine, Hori: "The inhibition of Bax activation-induced apoptosis by RasGRP2 via R-Ras-PI3K-Akt signaling pathway in the endothelial cells." in: Scientific reports, Vol. 9, Issue 1, pp. 16717, (2019) (PubMed).

Bera, Chiu, Huang, Liang, Lee, Shih: "Genetic and Epigenetic Perturbations by DNMT3A-R882 Mutants Impaired Apoptosis through Augmentation of PRDX2 in Myeloid Leukemia Cells." in: Neoplasia (New York, N.Y.), Vol. 20, Issue 11, pp. 1106-1120, (2019) (PubMed).

Dominguez, Maddelein, Pucelle, Nicaise, Maurage, Duyckaerts, Cuvillier, Delisle: "Neuronal sphingosine kinase 2 subcellular localization is altered in Alzheimer's disease brain." in: Acta neuropathologica communications, Vol. 6, Issue 1, pp. 25, (2019) (PubMed).

Li, Chen, Chen, Yen, Fang, Tsai, Chang, Shen, Huang, Chuu, Chang, Hsiung, Jiang: "Upregulation of CISD2 augments ROS homeostasis and contributes to tumorigenesis and poor prognosis of lung adenocarcinoma." in: Scientific reports, Vol. 7, Issue 1, pp. 11893, (2019) (PubMed).

Chakraborty, Tapryal, Venkova, Horikoshi, Pandita, Sarker, Sarkar, Pandita, Hazra: "Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes." in: Nature communications, Vol. 7, pp. 13049, (2018) (PubMed).

Liang, Shi, Yang, Qiu, Lin, Qi, Li, Zhao, Liu: "Inhibition of polypyrimidine tract-binding protein 3 induces apoptosis and cell cycle arrest, and enhances the cytotoxicity of 5- fluorouracil in gastric cancer cells." in: British journal of cancer, Vol. 116, Issue 7, pp. 903-911, (2017) (PubMed).

Xu, Wu, Zheng, Yu, Yang, Yao, Zhou, Ching, Lau: "Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells." in: Oncotarget, Vol. 7, Issue 5, pp. 6146-58, (2017) (PubMed).
抗原

PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

别名

poly(ADP-ribose) polymerase 1

背景

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Cellular Localization: Nucleus

分子量

113 kDa

基因ID

142

UniProt

P09874

途径

Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location