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PCNA 抗体

This anti-PCNA antibody is a 兔 多克隆 antibody detecting PCNA in WB, IHC, IF, IHC (p), IP, ICC, IHC (fro) 和 FISH. Suitable for 人. This Primary Antibody has been cited in 4+ publications.
产品编号 ABIN2854788
发货至: 中国

Quick Overview for PCNA 抗体 (ABIN2854788)

抗原

See all PCNA 抗体
PCNA (Proliferating Cell Nuclear Antigen (PCNA))

适用

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宿主

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克隆类型

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多克隆

标记

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This PCNA antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Fluorescence in situ hybridization (FISH)
  • 特异性

    IP/MS validation was supported by references (PMID:30377373)

    交叉反应

    Golden Syrian Hamster, 人, 小鼠, 大鼠

    产品特性

    Rabbit Polyclonal antibody to PCNA (proliferating cell nuclear antigen)
    PCNA antibody

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: NIH-3T3 , PC-12

    Validation: IP/MS, Orthogonal

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.11 mg/mL

    缓冲液

    1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Mousa, Alhumaydhi, Abdellatif, Abdulmonem, AlKhowailed, Alsagaby, Al Rugaie, Alnuqaydan, Aljohani, Aljasir, Alwashmi, Soliman, Yosof, Elsheikh, Babiker, Alsuhaibani, Hegazy, Seleem: "Curcumin and ustekinumab cotherapy alleviates induced psoriasis in rats through their antioxidant, anti-inflammatory, and antiproliferative effects." in: Cutaneous and ocular toxicology, pp. 1-10, (2021) (PubMed).

    Mousa, Soliman, Alhumaydhi, Almatroudi, Al Rugaie, Allemailem, Alrumaihi, Khan, Rezk, Aljasir, Alwashmi, Aba Alkhayl, Albutti, Seleem: "Garlic Extract Alleviates Trastuzumab-Induced Hepatotoxicity in Rats Through Its Antioxidant, Anti-Inflammatory, and Antihyperlipidemic Effects." in: Journal of inflammation research, Vol. 14, pp. 6305-6316, (2021) (PubMed).

    Chen, Chen, Lin, Hsieh, Hsu, Hsieh: "Synergistic Anticancer Effects of Gemcitabine with Pitavastatin on Pancreatic Cancer Cell Line MIA PaCa-2 in vitro and in vivo." in: Cancer management and research, Vol. 12, pp. 4645-4665, (2020) (PubMed).

    Hsieh, Wang, Changou, Chin, Yang, Lai, Lee, Yang, Whang-Peng, Liu, Lin, Mousa, Davis: "Crosstalk between integrin αvβ3 and ERα contributes to thyroid hormone-induced proliferation of ovarian cancer cells." in: Oncotarget, Vol. 8, Issue 15, pp. 24237-24249, (2018) (PubMed).

  • 抗原

    PCNA (Proliferating Cell Nuclear Antigen (PCNA))

    别名

    proliferating cell nuclear antigen

    背景

    The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

    Cellular Localization: Nucleus

    分子量

    29 kDa

    基因ID

    5111

    UniProt

    P12004

    途径

    Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
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