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PCNA 抗体

Name: PCNA 抗体
Brand: antibodies-online
SKU: ABIN2854788
Availability: OutOfStock

This anti-PCNA antibody is a 兔多克隆 antibody detecting PCNA in WB, IHC, IF, IHC (p), IP, ICC, IHC (fro) 和 FISH. Suitable for 人. This Primary Antibody has been cited in 4+ publications.

WB Image PCNA antibody detects PCNA protein by western blot analysis. (PCNA 抗体)

The effects of ustekinumab and CUC on PCNA IE in IQ-induced psoriatic skin lesions. (PCNA 抗体)

ISt

IHC-P Image PCNA antibody detects PCNA protein at nucleus on mouse testis by immunohistochemical analysis. (PCNA 抗体)

IHC

IHC-P Image PCNA antibody detects PCNA protein at nucleus on rat hind brain by immunohistochemical analysis. (PCNA 抗体)

IHC

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using PCNA, antibody (Green) at 1:500 dilution. (PCNA 抗体)

ICC/IF Image PCNA antibody detects PCNA protein at nucleus by immunofluorescent analysis. (PCNA 抗体)

IHC-P Image PCNA antibody detects PCNA protein at nucleus in human cervical carcinoma by immunohistochemical analysis. (PCNA 抗体)

IHC

IP Image Immunoprecipitation of PCBP2 protein from HeLa whole cell extracts using 5 μg of PCBP2 antibody, Western blot analysis was performed using PCBP2 antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent. (PCNA 抗体)

IHC-P Image PCNA antibody detects PCNA protein at nucleus on mouse liver by immunohistochemical analysis. (PCNA 抗体)

IHC

IHC-P Image PCNA antibody detects PCNA protein at nucleus in human colon by immunohistochemical analysis. (PCNA 抗体)

IHC

Lycogen™ reduced cisplatin-induced cell apoptosis. (PCNA 抗体)

Combination of bortezomib and citreoviridin inhibited cell cycle progression through different cell cycle regulators. (PCNA 抗体)

Thyroid hormone induced proliferation in ovarian cancer cellsOVCAR-3 and SKOV-3 were treated with T3 (10-9 to 10-7 M) or T4 (10-8 to 10-6 M) for 3 days (A), 24 hours (B, upper panel) or 30 min (B, lower panel). (PCNA 抗体)

Changes in important mediators of inflammation and fibrosis in the obstructed kidney 7 days after UUO and the effects of Sirt1 activation or inhibition. (PCNA 抗体)

Prostate tumors grown from Cav1-silenced PC3 cells were accompanied by a more reactive tumor stroma. (PCNA 抗体)

IHC (p)

16 images

(4 references)

产品编号 ABIN2854788

发货至: 中国

Quick Overview for PCNA 抗体 (ABIN2854788)

抗原

See all PCNA 抗体

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

适用

人

宿主

兔

克隆类型

多克隆

标记

This PCNA antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Fluorescence in situ hybridization (FISH)

Discover our top product PCNA Primary Antibody

特异性

IP/MS validation was supported by references (PMID:30377373)

交叉反应

Golden Syrian Hamster, 人, 小鼠, 大鼠

产品特性

Rabbit Polyclonal antibody to PCNA (proliferating cell nuclear antigen)
PCNA antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.

亚型

IgG
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 151-261) antibody
PCNA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 1G7 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal S1 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 53-196) antibody
PCNA 适用: 人, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 7H4F8 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 201-261) antibody
PCNA 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 6-261) antibody
PCNA 适用: 人, 小鼠, 大鼠, Pig, 犬, 兔, 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) antibody
PCNA 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1H10 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) antibody
PCNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 231-261), (C-Term) antibody
PCNA 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB17486 unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: NIH-3T3 , PC-12
Validation: IP/MS, Orthogonal

限制

仅限研究用
状态

Liquid

浓度

0.11 mg/mL

缓冲液

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Mousa, Alhumaydhi, Abdellatif, Abdulmonem, AlKhowailed, Alsagaby, Al Rugaie, Alnuqaydan, Aljohani, Aljasir, Alwashmi, Soliman, Yosof, Elsheikh, Babiker, Alsuhaibani, Hegazy, Seleem: "Curcumin and ustekinumab cotherapy alleviates induced psoriasis in rats through their antioxidant, anti-inflammatory, and antiproliferative effects." in: Cutaneous and ocular toxicology, pp. 1-10, (2021) (PubMed).

Mousa, Soliman, Alhumaydhi, Almatroudi, Al Rugaie, Allemailem, Alrumaihi, Khan, Rezk, Aljasir, Alwashmi, Aba Alkhayl, Albutti, Seleem: "Garlic Extract Alleviates Trastuzumab-Induced Hepatotoxicity in Rats Through Its Antioxidant, Anti-Inflammatory, and Antihyperlipidemic Effects." in: Journal of inflammation research, Vol. 14, pp. 6305-6316, (2021) (PubMed).

Chen, Chen, Lin, Hsieh, Hsu, Hsieh: "Synergistic Anticancer Effects of Gemcitabine with Pitavastatin on Pancreatic Cancer Cell Line MIA PaCa-2 in vitro and in vivo." in: Cancer management and research, Vol. 12, pp. 4645-4665, (2020) (PubMed).

Hsieh, Wang, Changou, Chin, Yang, Lai, Lee, Yang, Whang-Peng, Liu, Lin, Mousa, Davis: "Crosstalk between integrin αvβ3 and ERα contributes to thyroid hormone-induced proliferation of ovarian cancer cells." in: Oncotarget, Vol. 8, Issue 15, pp. 24237-24249, (2018) (PubMed).
抗原

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

别名

proliferating cell nuclear antigen

背景

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

Cellular Localization: Nucleus

分子量

29 kDa

基因ID

5111

UniProt

P12004

途径

Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy