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- 抗原 See all ITGA7 抗体
- ITGA7 (Integrin, alpha 7 (ITGA7))
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适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This ITGA7 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS)
- 交叉反应 （详细）
- Species reactivity (tested):Mouse.
- 纯化方法
- Protein-A Sepharose Chromatography.
- 免疫原
- Mouse myoblasts. Remarks: Hybridoma was established by fusion of mouse myeloma cell SP2/0 with Integrinalpha-7 knockout C57/B6 Mouse splenocyte.
- 克隆位点
- 3C12
- 亚型
- IgG1
- Top Product
- Discover our top product ITGA7 Primary Antibody
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anti-Integrin, alpha 7 (ITGA7) (AA 247-279), (N-Term) antibody
ITGA7 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB52834 unconjugated
anti-Integrin, alpha 7 (ITGA7) (AA 701-800) antibodyITGA7 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 7 (ITGA7) (AA 1149-1181), (C-Term) antibodyITGA7 适用: 人 WB 宿主: 兔 Polyclonal RB52832 unconjugated
anti-Integrin, alpha 7 (ITGA7) (AA 478-577) antibodyITGA7 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 8G2 unconjugated
anti-Integrin, alpha 7 (ITGA7) antibodyITGA7 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 7 (ITGA7) (AA 478-577) antibodyITGA7 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F4 unconjugated
anti-Integrin, alpha 7 (ITGA7) (Cleaved-Glu959) antibodyITGA7 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 7 (ITGA7) (AA 175-283) antibodyITGA7 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 7 (ITGA7) (AA 478-577) antibodyITGA7 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Integrin, alpha 7 (ITGA7) (AA 880-1020) antibodyITGA7 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Flow Cytometry: 25-50 μg/mL (final concentration). Positive Control: C2C12. Detailed Procedure is provided in the following Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Flow Cytometric Analysis for Floating CellsWe usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with washing buffer (5x10e6 cells/mL). 3) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute atroom temperature (20~25°C). Remove supernatant by careful aspiration. 4) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 5) Add 40 µL of the primary antibody at the concentration suggested in the APPLICATIONS,diluted in the washing buffer. Mix well and incubate for 30 minutes at room temperature. 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature. Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Positive Control for Flow Cytometry: C2C12
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- PBS containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- ITGA7 (Integrin, alpha 7 (ITGA7))
- 别名
- Integrin alpha-7 / ITGA7 (ITGA7 产品)
- 别名
- [a]7 antibody, alpha7 antibody, integrin alpha 7 antibody, integrin subunit alpha 7 antibody, Itga7 antibody, ITGA7 antibody
- 背景
- The integrin family of adhesion molecules participate in important cell-cell and cell-extracellular matrix interactions in a diverse range of biological processes. Integrins are heterodimers consisting of an alpha subunit and a beta subunit. Both alpha and beta subunits are transmembrane proteins with large extracellular domains (>100 kDa for alpha subunit and >75 kDa for beta subunit) that interact with extracellular matrix proteins and relatively small cytoplasmic domains (50 amino acids or less, except for the beta-4 subunit) that interact with cytoskeletal proteins. The adhesiveness of integrins is dynamically regulated in response to cytoplasmic signals, termed
- UniProt
- Q61738
- 途径
- Integrin Complex
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