Dinitrophenol 抗体
DNP
ELISA
宿主: 兔
Polyclonal
unconjugated
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- 抗原 See all Dinitrophenol (DNP) products
- Dinitrophenol (DNP)
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Dinitrophenol antibody is un-conjugated
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应用范围
- ELISA
- 免疫原
- DNP antibody was raised in rabbit using dinitrophenol-BSA as the immunogen.
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- 应用备注
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ELISA: 1:32,000
Optimal conditions should be determined by the investigator. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- Neat serum with 0.1 % NaN3.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 注意事项
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Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use. - 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
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- 抗原
- Dinitrophenol (DNP)
- 别名
- DNP (DNP 产品)
- 物质类
- Chemical
- 背景
- Tissues are continually exposed to reactive oxygen species (ROS) that are produced in tissues from metabolism processes or the inflammatory response of leukocytes or macrophages. These ROS can cause damage to various cellular components. Dinitrophenylhydrazine (DNPH) will react with free carbonyl groups on proteins, leading to adduction of the dinitrophenyl (DNP) group to the carbonyl group. Using an antibody to DNP, it is possible to visualize and localize the sites of oxidative damage by immunodetection of DNP adduction resulting from reaction of the tissue with DNPH.
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