Tested Western blot Application Notes Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.VPS37C antibody [N1], N-term detects VPS37C protein at cytoplasm by immunofluorescent analysis.Sample: Raji cells were fixed in 4 % paraformaldehyde at RT for 15 min.Green: VPS37C protein stained by VPS37C antibody [N1], N-term (107-10100) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 ?m.Sample(30 μg of whole cell lysate)A:Raji12 % SDS PAGE107-10100 diluted at 1:500
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
储存液
Thimerosal (Merthiolate)
注意事项
This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.