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LEF1 抗体 (N-Term)

The 兔多克隆 anti-LEF1 antibody is suitable to detect LEF1 in samples from 人, 小鼠, 大鼠, 犬, 豚鼠, Cow 和马. It has been validated for WB.

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产品编号 ABIN2792635

发货至: 中国

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Quick Overview for LEF1 抗体 (N-Term) (ABIN2792635)

抗原

See all LEF1 抗体

LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

适用

人, 小鼠, 大鼠, 犬, 豚鼠, Cow, 马

宿主

兔

克隆类型

多克隆

标记

This LEF1 antibody is un-conjugated

应用范围

Western Blotting (WB)

Discover our top product LEF1 Primary Antibody

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N-Term

序列

VARQAQTSQE PYHDKAREHP DDGKHPDGGL YNKGPSYSSY SGYIMMPNMN

交叉反应

牛, 人, 小鼠, 猪, 兔, 大鼠

预测反应

Cow: 92%, Dog: 92%, Guinea Pig: 85%, Horse: 100%, Human: 100%, Mouse: 85%, Rat: 92%

产品特性

This is a rabbit polyclonal antibody against LEF1. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the N terminal region of human LEF1
anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 100-399) antibody
LEF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 331-399) antibody
LEF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 1-399) antibody
LEF1 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 3H5 unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (Internal Region) antibody
Verified LEF1 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 33-138) antibody
LEF1 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 1A4B11 unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 14-123) antibody
LEF1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 5A3 unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 75-216) antibody
LEF1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 33-138) antibody
LEF1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1A8 unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 10-37), (N-Term) antibody
LEF1 适用: 人 WB, IF 宿主: 兔 Polyclonal RB31989 unconjugated

anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (Internal Region) antibody
LEF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB Suggested Anti-LEF1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: Hela cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 399 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
抗原

LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

别名

LEF1

背景

Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1). Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA, MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1, MIM 163905).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: DKFZp586H0919, TCF1ALPHA, LEF-1, TCF10, TCF7L3
Protein Interaction Partner: RNF138, UBC, RNF14, NLK, NARF, STAT5A, PITX2, CTNNB1, CDX1, DPYSL2, nrarp, FHIT, MITF, HINT1, TLE2, TLE1, SETD8, MYB, E2F1, HDAC1, ZBTB3, DACT1, DAZAP2, ALYREF, PIAS4, ALX4, SMAD3, SUMO2, EP300, SMAD1, SMAD2, SMAD4, KPNA1, KPNA2, UBTF, RUNX2, NOTCH1, RUVB
Protein Size: 399

分子量

44 kDa

基因ID

51176

NCBI登录号

NM_016269, NP_057353

UniProt

Q9UJU2

途径

WNT signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding