APAF1 抗体 (N-Term)
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北京 101111
Quick Overview for APAF1 抗体 (N-Term) (ABIN2745508)
抗原
See all APAF1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-464, N-Term
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原理
- anti-Apaf-1 (human), mAb (2E12)
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特异性
- Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.
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无交叉反应
- 小鼠, 大鼠
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交叉反应 （详细）
- Does not cross-react with mouse or rat Apaf-1.
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产品特性
- Monoclonal Antibody. Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1. Isotype: Rat IgG2akappa. Clone: 2.00E+12. Applications: ELISA, ICC, IHC, IP, WB. Liquid. In PBS containing 0.02 % sodium azide. Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
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纯度
- >95 % (SDS-PAGE)
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内毒素水平
- <0.1EU/μg purified protein (LAL test, Lonza).
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免疫原
- Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.
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亚型
- IgG2a kappa
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anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 1-250) antibody
APAF1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 1138-1237) antibodyAPAF1 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2H9A1 unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 31-350) antibodyAPAF1 适用: 人 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 13-80) antibodyAPAF1 适用: 人 IHC (p), IF (cc), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 1201-1248) antibodyAPAF1 适用: 人 WB, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (Internal Region) antibodyAPAF1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (C-Term) antibodyAPAF1 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 104-415) antibodyAPAF1 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (C-Term) antibodyAPAF1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 260-340) antibodyAPAF1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- In PBS containing 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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+4°C
-20°C
Stable for at least 1 year after receipt when stored at -20°C.
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有效期
- 12 months
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- APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))
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别名
- Apaf-1
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背景
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Alternate Names/Synonyms: Apoptosis Protease-activating Factor 1
Product Description: Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
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UniProt
- O14727
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途径
- p53 Pathway, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity
抗原
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