TDG 抗体
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北京 101111
Quick Overview for TDG 抗体 (ABIN272226)
抗原
See all TDG 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of TDG rotein. (region surrounding Lys90)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity Chromatography using epitope-specific immunogen.
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anti-Thymine-DNA Glycosylase (TDG) (AA 141-410) antibody
TDG 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Thymine-DNA Glycosylase (TDG) (AA 360-410) antibodyTDG 适用: 人 WB, IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-Thymine-DNA Glycosylase (TDG) (AA 1-410) antibodyTDG 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Thymine-DNA Glycosylase (TDG) (Internal Region) antibodyTDG 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Thymine-DNA Glycosylase (TDG) (AA 81-110), (N-Term) antibodyTDG 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB19893 unconjugated
anti-Thymine-DNA Glycosylase (TDG) (AA 281-310), (C-Term) antibodyTDG 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB19894 unconjugated
anti-Thymine-DNA Glycosylase (TDG) antibodyTDG 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Thymine-DNA Glycosylase (TDG) (AA 1-410) antibodyTDG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Thymine-DNA Glycosylase (TDG) (AA 1-160) antibodyTDG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Thymine-DNA Glycosylase (TDG) (AA 1-410) antibodyTDG 适用: 人 WB, ELISA, IF, ICC 宿主: 小鼠 Monoclonal AT2F7 unconjugated
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应用备注
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ELISA: 1/5000approx. 1/10000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50-1/200. Immunofluorescence: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- TDG (Thymine-DNA Glycosylase (TDG))
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别名
- TDG
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背景
- In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. G/T mismatch-specific Thymine DNA Glycosylase (TDG) is a nuclear protein which corrects G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymine. TDG also corrects a subset of G/U mispairs inefficiently removed by the more abundant uracil glycosylases. Retinoic acid receptors interact physically and functionally with TDG, enhancing the ability of the retinoid X receptor and the retinoid X receptor/retinoid acid receptor complex to bind to their response elements. TDG interacts with, and is covalently modified by, the ubiquitin-like proteins SUMO-1 and SUMO-2/3, resulting in a reduction of the DNA substrate and AP site binding affinity of TDG. This sumoylation is associated with a significant increase in enzymatic turnover in reactions with a G/U substrate and the loss of G/T processing activity.Synonyms: G/T mismatch-specific thymine DNA glycosylase
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分子量
- approx. 46 kDa
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基因ID
- 6996
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NCBI登录号
- NP_003202
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UniProt
- Q13569
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途径
- DNA Damage Repair, Chromatin Binding
抗原
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