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DGKI 抗体

DGKI 适用: 人, 大鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN272146
发货至: 中国
  • 抗原 See all DGKI 抗体
    DGKI (Diacylglycerol Kinase, iota (DGKI))
    适用
    • 43
    • 19
    • 11
    人, 大鼠
    宿主
    • 42
    • 1
    克隆类型
    • 42
    • 1
    多克隆
    标记
    • 14
    • 4
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This DGKI antibody is un-conjugated
    应用范围
    • 36
    • 26
    • 23
    • 13
    • 13
    • 7
    • 5
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    特异性
    This antibody detects endogenous levels of DGK-ι protein. (region surrounding Lys1023)
    交叉反应 (详细)
    Species reactivity (tested):Human, Rat.
    纯化方法
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
    纯度
    > 95 % pure by SDS-PAGE
    Top Product
    Discover our top product DGKI Primary Antibody
  • 应用备注
    ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1,0 mg/mL
    缓冲液
    Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    DO NOT FREEZE!
    储存条件
    4 °C
    储存方法
    Store the antibody undiluted at 2-8 °C.
  • 抗原
    DGKI (Diacylglycerol Kinase, iota (DGKI))
    别名
    DAG Kinase iota (DGKI 产品)
    别名
    DGK-IOTA antibody, rDGKi-3 antibody, C130010K08Rik antibody, diacylglycerol kinase iota antibody, diacylglycerol kinase, iota antibody, DGKI antibody, dgki antibody, LOC100560932 antibody, Dgki antibody
    背景
    Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-α (80 kDa), -β (90 kDa), and - γ (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-δ (130 kDa) and DGK-´(130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK-epsilon (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK-Ω (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-^θ (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.Synonyms: DGKI, Diacylglycerol kinase iota
    分子量
    approx. 118 kDa
    基因ID
    9162
    NCBI登录号
    NP_004708
    UniProt
    O75912
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