Vinculin 抗体
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- 抗原 See all Vinculin (VCL) 抗体
- Vinculin (VCL)
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Vinculin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody detects endogenous levels of Vinculin protein. (region surrounding Leu817)
- 交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- Affinity Chromatography using epitope-specific Immunogen.
- Top Product
- Discover our top product VCL Primary Antibody
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anti-Vinculin (VCL) (AA 1-1066) antibody
VCL 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C6-1B5 unconjugated
anti-Vinculin (VCL) (AA 879-1064) antibodyVCL 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vinculin (VCL) (AA 903-937) antibodyVCL 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB55983 unconjugated
anti-Vinculin (VCL) (AA 1020-1134) antibodyVCL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Vinculin (VCL) (AA 634-668) antibodyVCL 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB55982 unconjugated
anti-Vinculin (VCL) (AA 174-322) antibodyVCL 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal VCL-2572 unconjugated
anti-Vinculin (VCL) (AA 174-322) antibodyVCL 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal VCL-2575 unconjugated
anti-Vinculin (VCL) (AA 1-1066) antibodyVCL 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3F8-1D4 unconjugated
anti-Vinculin (VCL) (AA 767-1066) antibodyVCL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vinculin (VCL) antibodyVCL 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
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- 应用备注
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ELISA: 1/10000approx. 1/20000. Western Blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Vinculin (VCL)
- Abstract
- VCL 产品
- 别名
- CMD1W antibody, CMH15 antibody, MV antibody, MVCL antibody, VCL antibody, im:6902790 antibody, hm:zehn2160 antibody, DDBDRAFT_0186762 antibody, DDBDRAFT_0232320 antibody, DDB_0186762 antibody, DDB_0232320 antibody, 9430097D22 antibody, AA571387 antibody, AI462105 antibody, AW545629 antibody, CG3299 antibody, CT11081 antibody, Dmel\\CG3299 antibody, Dmvincp antibody, EG:103B4.1 antibody, Vin antibody, Vin2EF antibody, vinc antibody, LOC100228062 antibody, vcl-A antibody, vinculin antibody, vinculin a antibody, vinculin A antibody, Vinculin antibody, vinculin S homeolog antibody, VCL antibody, vcla antibody, vinA antibody, Vcl antibody, Vinc antibody, CpipJ_CPIJ013873 antibody, Bm1_37935 antibody, vcl antibody, vcl.S antibody
- 背景
- Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and α-actinin. In addition, vinculin, Talin and α-actinin each contain Actin binding sites. Expression of vinculin and Talin has been shown to be affected by the level of Actin expression. α-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.Synonyms: Metavinculin
- 分子量
- approx. 123 kDa
- 基因ID
- 7414
- NCBI登录号
- NP_003364
- UniProt
- P18206
- 途径
- Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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