PCNA 抗体
Our Local Distributor
北京 101111
Quick Overview for PCNA 抗体 (ABIN271780)
抗原
See all PCNA 抗体适用
宿主
克隆类型
标记
应用范围
-
-
交叉反应 (详细)
-
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
-
纯度
- > 95 % pure by SDS-PAGE
-
-
-
-
应用备注
-
ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 1,0 mg/mL
-
缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- DO NOT FREEZE!
-
储存条件
- 4 °C
-
储存方法
- Store the antibody undiluted at 2-8 °C.
-
-
- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
-
别名
- PCNA
-
背景
- The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication, and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli, however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms, one involved in ongoing DNA replication. which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.Synonyms: Cyclin, Proliferating Cell Nuclear Antigen
-
分子量
- approx. 29, 36 kDa
-
基因ID
- 9606
-
UniProt
- P12004
-
途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
抗原
-