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SLC1A3 抗体 (AA 522-541)

This independently validated 兔 多克隆 antibody specifically detects SLC1A3 in WB, IHC, IP 和 ICC. It exhibits reactivity toward 人, 大鼠 和 小鼠.
Independent Validation (1 validation)
产品编号 ABIN2690503
发货至: 中国
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中国
北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for SLC1A3 抗体 (AA 522-541) (ABIN2690503)

抗原

See all SLC1A3 抗体
SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

适用

  • 65
  • 56
  • 32
  • 4
  • 2
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  • 1
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人, 大鼠, 小鼠

宿主

  • 75
  • 4
  • 3
  • 2
兔

克隆类型

  • 66
  • 18
多克隆

标记

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This SLC1A3 antibody is un-conjugated

应用范围

  • 65
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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

质量等级

KO Validated

  • 抗原表位

    • 16
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    AA 522-541

    原理

    anti-EAAT1

    交叉反应 （详细）

    Reacts with: human (P43003), rat (P24942), mouse (P56564). Other species not tested yet.

    纯化方法

    affinity purified

    免疫原

    Synthetic peptide corresponding to AA 522 to 541 from rat EAAT1 (UniProt Id: P24942)
  • anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) antibody
    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) antibody

    SLC1A3 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated

    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 301-400) antibody
    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 301-400) antibody

    SLC1A3 适用: 人, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 14-42), (N-Term) antibody
    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 14-42), (N-Term) antibody

    SLC1A3 适用: 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated

    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 146-236) antibody
    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 146-236) antibody

    SLC1A3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 519-537), (C-Term) antibody
    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 519-537), (C-Term) antibody

    SLC1A3 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 188-200), (Extracellular) antibody
    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 188-200), (Extracellular) antibody

    SLC1A3 适用: 大鼠 WB, IHC, FACS, IF, LCI, IC 宿主: 兔 Polyclonal unconjugated

    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (Cytoplasmic Domain) antibody
    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (Cytoplasmic Domain) antibody

    SLC1A3 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (Cytoplasmic Domain) antibody
    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (Cytoplasmic Domain) antibody

    SLC1A3 适用: 人, 大鼠, 小鼠, 猴, 兔, Hamster, Cow, 马, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 1-542) antibody
    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 1-542) antibody

    SLC1A3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 162-237) antibody
    anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 162-237) antibody

    SLC1A3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D1 unconjugated

  • 应用备注

    WB:1 : 1000 up to 1 : 10000 , IP:yes ICC:1 : 1000 up to 1 : 5000, IHC:1 : 1000 up to 1 : 5000, IHC-P:not tested yet

    限制

    仅限研究用
  • 生效 #103349 (Western Blotting)
    '独立验证'标志
    by
    Department of Chemical Biology, Rutgers University
    No.
    #103349
    日期
    2018.08.29
    抗原
    SLC1A3
    Lot Number
    250113/3
    Method validated
    Western Blotting
    Positive Control

    Sut2 (xCT-/-), MASS20 (xCT+/+) and MelanA (xCT+/+) cells are all mouse melanocytes that express SLC1A3 transporter. Sut2 and MelanA were from Dr. D. Bennett in 1998 and have kept in our lab since then, MASS20 was made in our lab.

    Negative Control
    Notes

    Passed. ABIN2690503 does specifically recognize SLC1A3 in all three tested murine melanocyte extracts.

    '独立验证'标志
    Validation Images
    Western blot analysis with ABIN2690503 of Sut2 (SUT2), MASS20 (Mass20) and MelanA (MelanA) cells extracts and extracts from Sut2 cells transfected with xCT and/or Myc-Grm1. Image exposure 5s. Western blot analysis with ABIN2690503 of Sut2 (SUT2), MASS20 (Mass20) and MelanA (MelanA) cells extracts and extracts from Sut2 cells transfected with xCT and/or Myc-Grm1. Image exposure 5s.
    Full Methods
    Primary Antibody
    ABIN2690503
    Secondary Antibody
    HRP conjugated donkey anti rabbit antibody (Merck Millipore, AP182P, lot 3043559)
    Full Protocol
    • Grow Sut2 (xCT-/-), MASS20 (xCT+/+) and MelanA (xCT+/+) cells in RPMI medium (Sigma Aldrich, R8758, lot RNBG5596) supplemented with 10% fetal bovine serum (SAFC, 12303C, lot 16C364) and antibiotics (Penicillin-Streptomycin, Sigma Aldrich, 055M4784V, lot 097M4767V) at 37°C and 5% CO2 in 60mm tissue culture plates to 80% confluency. Supplement Sut2 cell lines 1M 2-Mercaptoethanol (Sigma Aldrich, SHBF4223V) and TPA (Sigma Aldrich, P1585) 0.1mg/ml. Supplement MelanA cells with TPA.
    • Using 1x Laemmli buffer (4x Laemmli Sample Buffer (Bio-Rad, 161-0747, lot 64098129) containing 20%/vol 2-Mercaptoethanol (Sigma Aldrich, SHBF4223V), diluted 1:4 in PBS).
    • Collect protein lysates using a cell scraper.
    • Heat lysates in a glycerol block for 10min at 99°C. Centrifuge lysates at 14000rpm for 10min at 4°C. Collect supernatant in a 1.5ml microcentrifuge tube and store at -80°C.
    • Thaw samples on ice and transfer 20µl of each lysate to a 0.5ml microcentrifuge tube.
    • Heat lysates in a glycerol block for 5min at 95°C and place them on ice immediately.
    • Separate samples on a freshly cast denaturing 10% SDS-PAGE in a Bio-Rad Mini PROTEAN 3 cell (serial number 525BR 047264) for 2h at 105V alongside protein ladder (Bio-Rad, 61-0374).
    • Transfer proteins in transfer buffer (made in house) onto nitrocellulose membrane (GVS Life Sciences, 1215471, lot number 7051342) in another Bio-Rad Mini PROTEAM 3 cell (serial number 525BR 047264) for 2h at 4°C at 160mA.
    • Confirm successful protein transfer using Ponceau red.
    • Block protein using 100ml blocking buffer (transfer buffer containing 0.25% milk) in a Snap i.d. 2.0 (Merck Millipore, C73105).
    • Incubate with primary rabbit anti-SLC1A3 antibody (antibodies-online, ABIN2690503, lot 250113/3) diluted 1:10000 and rabbit anti-GAPDH antibody (Santa Cruz Biotechnology, 25778) diluted 1:200 in blocking buffer ON at 4°C.
    • Wash membrane 5x in a Snap i.d. 2.0 with 1x TBST.
    • Incubation with secondary HRP conjugated donkey anti rabbit antibody (Merck Millipore, AP182P, lot 3043559) diluted 1:5000 in 0.25% milk (powdered goat milk rehydrated in 1X TBST) for 1h at RT.
    • Wash membrane 5x in a Snap i.d. 2.0 with 1x TBST.
    • Incubate membrane with 2ml Luminata Forte Western HRP Substrate (Merck Millipore, WBLUF0100, 180333) for 3min at RT. Reveal protein bands on a Syngene Gel Documentation system at 5s, 15s, and 30second exposures.
    Experimental Notes

    • ABIN2690503 reveals a band of the expected 60kDa MW in each of the three tested melanocyte extracts. Cell lines transfected with both xCT and Myc-Grm1 appear to have a double band at 60kDa.

    • SLC1A3 did not appear to be upregulated in the xCT-/- cell line SUT2.

  • 状态

    Lyophilized

    溶解方式

    For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

    缓冲液

    PBS. Albumin was added for stabilization.

    注意事项

    Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

    储存条件

    -20 °C,-80 °C,4 °C

    储存方法

    Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin was added for stabilization. Do not freeze!

  • 抗原

    SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

    别名

    EAAT1

    背景

    GLAST, SLC1A3, Glutamate Transporter, Neuroinflamm-Astrocyt, GLAST-1

    UniProt

    P24942

    途径

    Sensory Perception of Sound, Synaptic Membrane, Dicarboxylic Acid Transport
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