SLC1A3 抗体 (Cytoplasmic Domain)
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- 抗原 See all SLC1A3 抗体
- SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))
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抗原表位
- Cytoplasmic Domain
- 适用
- 人, 大鼠, 小鼠, 猴, Hamster, Cow, 马, Pig, 兔
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SLC1A3 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human SLC1A3 / GLAST. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 预测反应
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Horse, Rabbit, Pig (100%) Panda, Dog, Opossum, Guinea pig, Turkey, Chicken (95%) Xenopus (84%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic 19 amino acid peptide from cytoplasmic domain of human SLC1A3 / GLAST. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Horse, Rabbit, Pig (100%), Panda, Dog, Opossum, Guinea pig, Turkey, Chicken (95%), Xenopus (84%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product SLC1A3 Primary Antibody
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anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (Cytoplasmic Domain) antibody
SLC1A3 适用: 人, 大鼠, 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) antibodySLC1A3 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 301-400) antibodySLC1A3 适用: 人, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 14-42), (N-Term) antibodySLC1A3 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 146-236) antibodySLC1A3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 7-151) antibodySLC1A3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 519-537), (C-Term) antibodySLC1A3 适用: 大鼠, 小鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (2nd Extracellular Loop), (AA 188-200) antibodySLC1A3 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 1-542) antibodySLC1A3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 162-237) antibodySLC1A3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D1 unconjugated
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- 应用备注
- Approved: IHC, IHC-P (5 μg/mL)
- 说明
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Target Species of Antibody: Human
- 实验流程
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, less than 0.1 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))
- 别名
- SLC1A3 / GluT-1 / GLAST (SLC1A3 产品)
- 别名
- AF001784 antibody, CG3747 antibody, Dglt-1 antibody, Dglut-1 antibody, Dmel\\CG3747 antibody, EAAT antibody, EAAT1 antibody, dEAAT antibody, dEAAT1 antibody, dEaat1 antibody, slc1a3 antibody, wu:fb98e05 antibody, wu:fd50b05 antibody, zgc:56661 antibody, zgc:77768 antibody, SLC1A3 antibody, GLAST antibody, GluT-1 antibody, EA6 antibody, GLAST1 antibody, AI504299 antibody, B430115D02Rik antibody, Eaat1 antibody, GLAST-1 antibody, GLU-T antibody, Gmt1 antibody, MGluT1 antibody, Excitatory amino acid transporter 1 antibody, solute carrier family 1 member 3 antibody, excitatory amino acid transporter 1 antibody, solute carrier family 1 (glial high affinity glutamate transporter), member 3a antibody, solute carrier family 1 (glial high affinity glutamate transporter), member 3 antibody, Eaat1 antibody, SLC1A3 antibody, PTRG_00379 antibody, Tsp_02392 antibody, slc1a3a antibody, Slc1a3 antibody
- 背景
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Name/Gene ID: SLC1A3
Subfamily: Sodium:dicarboxylate symporter
Family: Transporter
Synonyms: SLC1A3, EA6, EAAT-1, EAAT1, GLAST, GLAST1, GLAST-1, GLUT-1 - 基因ID
- 6507
- 途径
- Sensory Perception of Sound, Synaptic Membrane, Dicarboxylic Acid Transport
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