(12 references)-
- 抗原
- T Cell Receptor (TCR) V beta 6
- 适用
- 小鼠
- 宿主
- 大鼠
- 克隆类型
- 单克隆
- 标记
- Biotin
- 应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The RR4-7 antibody reacts with the Vβ 6 T-Cell Receptor (TCR) of mice having the a (e.g., C57BR, C57L, SJL) and b (e.g., A, BALB/c, CBA/Ca, C3H/He, C57BL, DBA/1) haplotypes of the Tcrb gene complex. The Tcrb-V6 gene locus is deleted in mice having the c (e.g., RIII) haplotype. Vβ 6 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by Mtv-7 (Mls-1[a], Mls[a]) endogenous provirus (e.g., AKR, CBA/J, C58, DBA/2, NZB), or Mtv-43 endogenous provirus (e.g., MA/MyJ). Exogenous MMTV-SW, as well as endogenous Mtv-44-encoded superantigen (e.g., NZW), also causes incomplete elimination of Vβ 6 TCR-expressing T cells. Plate-bound RR4-7 antibody activates Vβ 6 TCR-bearing T cells, soluble RR4-7 mAb blocks in vitro proliferation and cytolytic activities of Vβ 6 TCR-bearing T-cell clones, and injection of the antibody results in in vivo depletion of Vβ 6 TCR-bearing T cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of Vβ 6 TCR on peripheral T lymphocytes. C57BL/6 lymph nodes cells were incubated simultaneously with biotin-conjugated RR4-7 (right panel), PE-conjugated RM4-5 (anti-mouse CD4, Cat. No. 553048/553049, both panels), and PE-conjugated 53-6.7 (anti-mouse CD8a, Cat. No. 553032/553033, both panels) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057, both panels). Flow cytometry was performed on a FACScan™ .
BD Pharmingen™ Biotin Rat Anti-Mouse Vβ 6 T-Cell Receptor - Biotin - Clone RR4-7 - Isotype Rat IgG2b, λ - Reactivity Ms - 0.25 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- C57BL/6 Mouse Helper T-Cell
- 克隆位点
- RR4
- 亚型
- IgG2b lambda
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "An exogenous mouse mammary tumor virus with properties of Mls-1a (Mtv-7)." in: The Journal of experimental medicine, Vol. 175, Issue 6, pp. 1623-33, (1992) (PubMed).
: "Mls-1-like superantigen in the MA/MyJ mouse is encoded by a new mammary tumor provirus that is distinct from Mtv-7." in: The Journal of experimental medicine, Vol. 175, Issue 6, pp. 1613-21, (1992) (PubMed).
: "Positive and negative selection of Tcrb-V6+ T cells." in: Immunogenetics, Vol. 36, Issue 4, pp. 230-7, (1992) (PubMed).
: "The V beta repertoire of mouse gut homodimeric alpha CD8+ intraepithelial T cell receptor alpha/beta + lymphocytes reveals a major extrathymic pathway of T cell differentiation." in: The Journal of experimental medicine, Vol. 173, Issue 2, pp. 483-6, (1991) (PubMed).
: "Extrathymic tolerance of mature T cells: clonal elimination as a consequence of immunity." in: Cell, Vol. 63, Issue 6, pp. 1249-56, (1991) (PubMed).
: "Peripheral clonal elimination of functional T cells." in: Science (New York, N.Y.), Vol. 250, Issue 4988, pp. 1726-9, (1991) (PubMed).
: "Differential reactivity of V beta 9 T cells to minor lymphocyte stimulating antigen in vitro and in vivo." in: European journal of immunology, Vol. 21, Issue 4, pp. 1007-11, (1991) (PubMed).
: "Failure of clonal deletion in neonatally thymectomized mice: tolerance is preserved through clonal anergy." in: The Journal of experimental medicine, Vol. 172, Issue 5, pp. 1277-85, (1990) (PubMed).
: "A nondeletional mechanism of thymic self tolerance." in: Science (New York, N.Y.), Vol. 246, Issue 4933, pp. 1038-41, (1990) (PubMed).
: "RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes." in: The Journal of experimental medicine, Vol. 169, Issue 6, pp. 1903-9, (1989) (PubMed).
: "The T cell receptor V beta 6 domain imparts reactivity to the Mls-1a antigen." in: Cellular immunology, Vol. 119, Issue 2, pp. 412-26, (1989) (PubMed).
: "In vivo T cell tumor therapy with monoclonal antibody directed to the V beta chain of T cell antigen receptor." in: The Journal of experimental medicine, Vol. 170, Issue 5, pp. 1513-9, (1989) (PubMed).
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: "An exogenous mouse mammary tumor virus with properties of Mls-1a (Mtv-7)." in: The Journal of experimental medicine, Vol. 175, Issue 6, pp. 1623-33, (1992) (PubMed).
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- 抗原
- T Cell Receptor (TCR) V beta 6
- 别名
- Vbeta 6 T-Cell Receptor
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