我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

CD14 抗体

Name: CD14 抗体
SKU: ABIN2689901
Availability: OutOfStock

This 大鼠单克隆 antibody specifically detects CD14 in ELISA. It exhibits reactivity toward 人. It has been mentioned in 9+ publications

(9 references)

产品编号 ABIN2689901

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CD14 抗体 (ABIN2689901)

抗原

See all CD14 抗体

CD14

适用

人

宿主

大鼠

克隆类型

单克隆

标记

This CD14 antibody is un-conjugated

应用范围

ELISA

克隆位点

Discover our top product CD14 Primary Antibody

产品特性

The 55-3 monoclonal antibody reacts with human CD14. CD14 is a 53 - 55 kDa cell surface glycoprotein that is expressed by monocytes, macrophages, and activated granulocytes as a glycophosphatidylinositol-linked protein. CD14 functions as a receptor for lipopolysaccharide (LPS, endotoxin) when LPS is bound to LBP (LPS binding protein). Soluble forms of CD14 (sCD14) can be detected either in serum or in supernatants from cell cultures. Soluble CD14 is produced either by shedding of membrane CD14 or by release from cells before addition of the GPI anchor. Both membrane and sCD14 function to enhance cell activation by LPS. Binding of LPS to CD14 activates monocytes or macrophages to produce cytokines and express adhesive proteins and low molecular weight proinflammatory mediators. Non-CD14 expressing cells, such as endothelial cells, can be activated by a complex of LPS and sCD14. Serum levels of sCD14 increase in association with sepsis, infectious disease, autoimmunity, and allergic disorders. The gene for human CD14 has been cloned and sequenced and recombinant sCD14 has been shown to be protective against LPS-induced lethality. The immunogen used to generate the 55-3 hybridoma was purified, soluble recombinant human CD14 protein.

BD Pharmingen™ Purified Rat Anti-Human sCD14 - Purified - Clone 55-3 - Isotype Rat IgG2a, κ - Reactivity Hu - 0.5 mg

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human CD14 Recombinant Protein

亚型

IgG2a kappa
anti-CD14 (CD14) (AA 20-214) antibody
CD14 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4B4F12 unconjugated

anti-CD14 (CD14) (AA 20-214) antibody
CD14 适用: 人 IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1H5D8 unconjugated

anti-CD14 (CD14) (AA 54-83), (N-Term) antibody
CD14 适用: 人 FACS, WB, IF, IHC (p) 宿主: 兔 Polyclonal RB14105 unconjugated

anti-CD14 (CD14) (AA 25-148) antibody
CD14 适用: 人 FACS, WB, IHC 宿主: 小鼠 Monoclonal LPSR-2385 unconjugated

anti-CD14 (CD14) antibody
CD14 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 1A3B6 unconjugated

anti-CD14 (CD14) (AA 20-345) antibody
CD14 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 14G1F3 unconjugated

anti-CD14 (CD14) (AA 230-345) antibody
CD14 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 1D1F8 unconjugated

anti-CD14 (CD14) (AA 65-327) antibody
CD14 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-CD14 (CD14) (AA 201-300) antibody
CD14 适用: 人, 小鼠, 大鼠, 犬, 绵羊, 兔 WB, ELISA, ICC, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-CD14 (CD14) (Internal Region) antibody
Verified CD14 适用: 人 FACS, WB, ELISA 宿主: 山羊 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

Aqueous buffered solution containing ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store undiluted at 4°C.
Egerer, Feist, Rohr, Pruss, Burmester, Dörner: "Increased serum soluble CD14, ICAM-1 and E-selectin correlate with disease activity and prognosis in systemic lupus erythematosus." in: Lupus, Vol. 9, Issue 8, pp. 614-21, (2000) (PubMed).

Nockher, Wick, Pfister: "Cerebrospinal fluid levels of soluble CD14 in inflammatory and non-inflammatory diseases of the CNS: upregulation during bacterial infections and viral meningitis." in: Journal of neuroimmunology, Vol. 101, Issue 2, pp. 161-9, (1999) (PubMed).

Haziot, Rong, Lin, Silver, Goyert: "Recombinant soluble CD14 prevents mortality in mice treated with endotoxin (lipopolysaccharide)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 12, pp. 6529-32, (1995) (PubMed).

Ulevitch, Tobias: "Receptor-dependent mechanisms of cell stimulation by bacterial endotoxin." in: Annual review of immunology, Vol. 13, pp. 437-57, (1995) (PubMed).

Arditi, Zhou, Dorio, Rong, Goyert, Kim: "Endotoxin-mediated endothelial cell injury and activation: role of soluble CD14." in: Infection and immunity, Vol. 61, Issue 8, pp. 3149-56, (1993) (PubMed).

Haziot, Rong, Silver, Goyert: "Recombinant soluble CD14 mediates the activation of endothelial cells by lipopolysaccharide." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 3, pp. 1500-7, (1993) (PubMed).

Ferrero, Goyert: "Nucleotide sequence of the gene encoding the monocyte differentiation antigen, CD14." in: Nucleic acids research, Vol. 16, Issue 9, pp. 4173, (1988) (PubMed).

Haziot, Chen, Ferrero, Low, Silber, Goyert: "The monocyte differentiation antigen, CD14, is anchored to the cell membrane by a phosphatidylinositol linkage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 2, pp. 547-52, (1988) (PubMed).

Goyert, Ferrero, Rettig, Yenamandra, Obata, Le Beau: "The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors." in: Science (New York, N.Y.), Vol. 239, Issue 4839, pp. 497-500, (1988) (PubMed).
抗原

CD14

别名

sCD14

背景

Synonyms: Soluble CD14

途径

TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades