(2 references)-
- 抗原 See all IL-8 (IL8) 抗体
- IL-8 (IL8) (Interleukin 8 (IL8))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This IL-8 antibody is un-conjugated
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应用范围
- Immunocytochemistry (ICC)
- 品牌
- BD Pharmingen™
- 产品特性
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The G265-8 antibody reacts with both the 72 and 77 amino acid forms of human interleukin-8 (IL-8). The immunogen used to produce the G265-8 hybridoma was E. coli-expressed recombinant human IL-8. PBMC were isolated from human peripheral blood by density gradient centrifugation and were cultured overnight at 37 °C with LPS (Sigma No. L-8274, 1 μg / ml) in the presence of GolgiStop™ (Cat. No. 554724). The activated cells were harvested and the level of IL-8 producing cells was detected by immunocytochemistry using a three-step staining procedure that employs a Biotin Goat anti-mouse IgG secondary antibody (Cat. No. 550337) and Streptavdin-horseradish peroxidase (Cat. No. 550946) (Nomarski optics, original magnification 400X).
BD Pharmingen™ Purified Mouse Anti-Human IL-8 - Purified - Clone G265-8 - Isotype Mouse IgG2b - Reactivity Hu - 0.25 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Recombinant Human IL-8
- 克隆位点
- G265
- 亚型
- IgG2b
- Top Product
- Discover our top product IL8 Primary Antibody
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anti-Interleukin 8 (IL8) (AA 23-101) antibody
IL8 适用: 马 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (AA 21-99) antibodyIL8 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) antibodyIL8 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal unconjugated
anti-Interleukin 8 (IL8) (AA 72-99) antibodyIL8 适用: 人 WB, ELISA, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) antibodyIL8 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (AA 72-99), (C-Term) antibodyIL8 适用: 人 WB, FACS, IHC (p), IF 宿主: 兔 Polyclonal RB20676 unconjugated
anti-Interleukin 8 (IL8) (AA 23-101) antibodyIL8 适用: 犬 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (AA 23-101) antibodyIL8 适用: 犬 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Interleukin 8 (IL8) (AA 28-99) antibodyIL8 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 8 (IL8) (C-Term) antibodyIL8 适用: 人 WB, ELISA, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验流程
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FOR IMMUNOCYTOCHEMICAL STAINING OF SINGLE-CELL PREPARATIONS This procedure describes the immunoenzymatic technique of staining cytokine within individual cells that are immobilized on microscopic slides via adherence (adherent slides) or centrifugation (cytospins). ADHESION SLIDES 1. Harvest cells and wash them twice in PBS using centrifugation (400 x g for 5 min) to remove residual protein. 2. Adjust the cell concentration at 4-5 x 10e6 cells/mL in PBS. 3. Place 20 μL of the cell suspension in each well of the adhesion slides and let them adhere at room temperature (RT) for 20 min. Please note that the slides should be washed in PBS at RT for 5 min before transferring the cells. 4. Fix cells on slides using fixation buffer for 15 min at RT. 5. Wash slides 2X in PBS with 5 min incubations. 6. Block slides with PBS supplemented with 1 % (w/v) BSA (Sigma, Cat. No. A43-78) for 30 min at RT or 10 min at 37 °C. 7. Wash slides 2X in PBS and proceed with staining or air dry them and store them at -80 °C for future use. 8. Incubate slides with 20 il of 1 % goat serum and PBS with 0.1 % (w/v) saponin for 30 min at RT. 9. Wash slides 2X with PBS with 5 min incubations. 10. Block endogenous peroxidase activity with Endogenous Peroxidase Blocking Buffer (20 μL/well) for 10 min at RT. 11. Wash 2X in PBS with 5 min incubations. 12. Incubate each well with Avidin (20 μL/well) for 15 min. 13. Wash 2X in PBS with 5 min incubations. 14. Incubate each well with Biotin (20 μL/well) for 15 min. 15. Wash 2X in PBS with 5 min incubations. 16. Incubate each well for 1 hr at RT with 20 μL of purified cytokine-specific antibody or appropriate immunoglobulin isotype control diluted in Antibody Diluent for IHC supplemented with saponin. 17. Wash slides 2X in PBS with 5 min incubations. 18. Incubate each well with 20 μL of a biotinylated secondary antibody diluted in Antibody Diluent for IHC for 30 min at RT. 19. Wash 2X in PBS with 5 min incubations. 20. Apply 20 μL of Streptavidin-HRP (BD Cat. No. 550946) to each well on slides and incubate for 30 min at RT. 21. Wash slides 2X with PBS with 5 minutes incubations. 22. Incubate with DAB Substrate as directed, (BD Cat. No. 550880) for less than 5 min at RT. 23. Stop the development of the color reaction by washing with PBS. 24. The slides are subsequently mounted in short-term storage mounting medium. CYTOSPINS 1. Assemble the Cytospin's sample chamber (e.g. Cytospin 3, Shandon, UK or comparable centrifuge), filter card, slide and cytospin racks according to manufacturer's specifications. 2. Load 40 μL of approximately 1 x 10e6 cells to each sample chamber. 3. Spin slides at 600 rpm for 2 min. 4. Take slides out of the cytospin rack and place them on a staining rack. 5. For fixation and staining please follow the steps 4 through 24 specified above for staining cells on adhesion slides.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF." in: Cytokine, Vol. 1, Issue 1, pp. 2-13, (1991) (PubMed).
: "Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 29, Issue 4, pp. 577-80, (1981) (PubMed).
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: "Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF." in: Cytokine, Vol. 1, Issue 1, pp. 2-13, (1991) (PubMed).
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- 抗原
- IL-8 (IL8) (Interleukin 8 (IL8))
- 别名
- IL-8 (IL8 产品)
- 别名
- CXCL8 antibody, GCP-1 antibody, GCP1 antibody, LECT antibody, LUCT antibody, LYNAP antibody, MDNCF antibody, MONAP antibody, NAF antibody, NAP-1 antibody, NAP1 antibody, IL-8 antibody, AMCF-I antibody, il8 antibody, IL8 antibody, si:dkey-151b16.2 antibody, cxcli2 antibody, cxcl8 antibody, gcp-1 antibody, il-8 antibody, lynap antibody, mdncf antibody, monap antibody, nap-1 antibody, C-X-C motif chemokine ligand 8 antibody, interleukin 8 antibody, chemokine (C-X-C motif) ligand 8a antibody, chemokine (C-X-C motif) ligand 1-like antibody, chemokine (C-X-C motif) ligand 8 L homeolog antibody, CXCL8 antibody, il8 antibody, cxcl8a antibody, IL8 antibody, Cxcl8 antibody, LOC422654 antibody, cxcl8.L antibody
- 途径
- TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy
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