EMR4 抗体
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- 抗原 See all EMR4 抗体
- EMR4 (EGF-Like Module Containing, Mucin-Like, Hormone Receptor-Like Sequence 4 (EMR4))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This EMR4 antibody is un-conjugated
- 应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The 6F12 antibody reacts with a 7-transmembrane-domain protein, which is similar to the F4/80 macrophage antigen of the EGF-TM7 protein family and is encoded by the Emr4 gene. The FIRE protein is expressed on myeloid cells with a denditic cell (DC) developmental potential, including subsets of DC and macrophages in the spleen and lymph nodes, most resident peritoneal macrophages, many peripheral blood monocytes, and a subpopulation of bone-marrow myeloid-cell progenitors. The protein is not detected on peripheral T and B lymphocytes, and it is down-regulated on thioglycollate-elicited peritoneal macrophages and on dendritic cells activated by GM-CSF, IFN-γ, anti-CD40, and LPS. Using soluble biotinylated fusion protein, a FIRE ligand was detected on a mouse IgG+ B lymphoma cell line (A20), but not on myeloid, fibroblast, or T-cell lines, suggesting that the FIRE protein may be involved in immunoregulatory interactions between antigen-presenting cells and B lymphocytes. Three-analysis of the expression of FIRE on bone-marrow leukocytes. BALB/c bone-marrow leukocytes were stained with either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553297, left panels) or purified mAb 6F12 (right panels), in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142), followed by biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894), then Streptavidin-APC (Cat. No. 554067). Myeloid cells were identified by staining with FITC-conjugated anti-mouse CD11b mAb M1/70 (Cat. No. 557396/553310, upper panels)and dendritic cells were identified with PE-conjugated anti-mouse Cd11c mAb HL3 (Cat. No. 557401/553802, bottom panels). The right panels demonstrate that the hematopoietic precursors which express FIRE protein are CD11b[intermediate]CD11c-. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.
BD Pharmingen™ Purified Rat Anti-Mouse F4/80-Like Receptor - Purified - Clone 6F12 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- CHO cells expressing recombinant FIRE fusion protein
- 克隆位点
- 6F12
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product EMR4 Primary Antibody
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anti-EGF-Like Module Containing, Mucin-Like, Hormone Receptor-Like Sequence 4 (EMR4) (Internal Region) antibody
EMR4 适用: 人, 猴 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-EGF-Like Module Containing, Mucin-Like, Hormone Receptor-Like Sequence 4 (EMR4) antibodyEMR4 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-EGF-Like Module Containing, Mucin-Like, Hormone Receptor-Like Sequence 4 (EMR4) (AA 101-150) antibodyEMR4 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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- 抗原
- EMR4 (EGF-Like Module Containing, Mucin-Like, Hormone Receptor-Like Sequence 4 (EMR4))
- 别名
- F4/80-Like Receptor (EMR4 产品)
- 别名
- D17Ertd479e antibody, Egf-tm7 antibody, Fire antibody, Gpr127 antibody, adhesion G protein-coupled receptor E4 antibody, Adgre4 antibody
- 背景
- Synonyms: FIRE
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