(3 references)-
- 抗原 See all CD200 抗体
- CD200
-
适用
- 小鼠
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- This CD200 antibody is un-conjugated
-
应用范围
- Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA
- 品牌
- BD Pharmingen™
- 产品特性
-
The OX-90 antibody reacts with CD200 (OX-2 antigen), a type-1 membrane glycoprotein containing two extracellular Ig-like domains. CD200 is expressed on thymocytes, neurons, B lymphocytes, splenic follicular dendritic cells and endothelium, and subsets of T lymphocytes and dendritic cells, but not on NK cells, granulocytes, monocytes, or macrophages. The CD200 Receptor is restricted to myeloid cells, and it is believed that its engagement with CD200 results in inhibition and/or down-regulation of myeloid cell activity. Multi-color analysis of the expression of CD200 on thymocytes. C57BL/6 thymocytes were stained with either purified mAb OX-90 (open histograms) or purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553927, shaded histograms), followed by biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894) then Streptavidin-PE (Cat. No. 554061). Then the cells were stained with FITC-conjugated anti-mouse CD4 mAb and APC-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553035). Electronic gates were set to include the CD4-CD8a-, CD4+CD8a+, CD4+CD8a-, and CD4-CD8a+ populations, and their expression of CD200 is displayed in the corresponding panels. Flow cytometry was performed on a BD FACSCalibur™
BD Pharmingen™ Purified Rat Anti-Mouse CD200 - Purified - Clone OX-90 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Soluble fusion protein of the extracellular region of mouse OX-2 antigen w/domains 3 & 4 of rat CD4
- 克隆位点
- OX
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product CD200 Primary Antibody
-
anti-CD200 (CD200) (Internal Region) antibody
CD200 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD200 (CD200) (AA 41-140) antibodyCD200 适用: 人 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD200 (CD200) (AA 31-232) antibodyCD200 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD200 (CD200) antibody (PE)CD200 适用: 人 FACS 宿主: 小鼠 Monoclonal OX-104 PE
anti-CD200 (CD200) antibody (FITC)CD200 适用: 人 FACS 宿主: 小鼠 Monoclonal OX-104 FITC
anti-CD200 (CD200) antibodyCD200 适用: 人 FACS, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal OX-104 unconjugated
anti-CD200 (CD200) (AA 56-257) antibodyCD200 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6E8B11 unconjugated
anti-CD200 (CD200) (AA 31-232) antibodyCD200 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM156 unconjugated
anti-CD200 (CD200) antibody (APC)CD200 适用: 人 FACS 宿主: 小鼠 Monoclonal OX-104 APC
anti-CD200 (CD200) antibodyCD200 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
- Flow Cytometry System.
- 限制
- 仅限研究用
-
- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
-
-
: "Down-regulation of the macrophage lineage through interaction with OX2 (CD200)." in: Science (New York, N.Y.), Vol. 290, Issue 5497, pp. 1768-71, (2000) (PubMed).
: "Evidence that an OX-2-positive cell can inhibit the stimulation of type 1 cytokine production by bone marrow-derived B7-1 (and B7-2)-positive dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 2, pp. 774-81, (1999) (PubMed).
: "The leukocyte/neuron cell surface antigen OX2 binds to a ligand on macrophages." in: European journal of immunology, Vol. 27, Issue 8, pp. 1911-8, (1997) (PubMed).
-
: "Down-regulation of the macrophage lineage through interaction with OX2 (CD200)." in: Science (New York, N.Y.), Vol. 290, Issue 5497, pp. 1768-71, (2000) (PubMed).
-
- 抗原
- CD200
- 别名
- CD200 (CD200 产品)
- 别名
- MOX1 antibody, MOX2 antibody, MRC antibody, OX-2 antibody, CD200 antibody, Cspmo2 antibody, MRCOX2 antibody, Mox2 antibody, DKFZp459C0716 antibody, OX2 antibody, CD200 molecule antibody, Cd200 molecule antibody, CD200 antigen antibody, CD200 antibody, Cd200 antibody
- 背景
- Synonyms: OX-2 Antigen
-
You are here:
