(7 references)-
- 抗原 See all ITGAL 抗体
- ITGAL (Integrin alpha-L (ITGAL))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This ITGAL antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- BD Pharmingen™
- 产品特性
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The M17/4 antibody reacts with the 180 kDa αL chain of LFA-1 (CD11a/CD18, αLβ2 integrin), a heterodimeric surface glycoprotein expressed on almost all leukocytes. CD8a+CD8b- intestinal intraepithelial T lymphocytes, which are believed to be thymus independent, do not express CD11a. LFA-1 mediates a variety of heterotypic and homotypic intercellular adhesions through interaction with ICAM-1 (CD54) and ICAM-2 (CD102), including participation in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. mAb M17/4 blocks a variety of LFA-1-mediated cells interactions in vitro, and costimulatory effects have also been described. In vivo treatment with M17/4 mAb reduces the severity of graft-versus-host reactions, prolongs allograft survival, inhibits the development of autoimmunity, and blocks substance P-induced leukocyte migration. The M17/4 and 2D7 (Cat. No. 553120) antibodies are reported to recognize different epitopes of the CD11a molecule. Immunohistochemical staining of CD11a positive cells. Frozen sections of mouse spleen were reacted with the anti-CD11a antibody. Leukocytes can be identified by the brown labeling of their cell membranes. Amplification 20X.
BD Pharmingen™ Purified Rat Anti-Mouse CD11a - Purified - Clone M17/4 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- C57BL/6 Mouse Splenic Secondary Cytotoxic T Lymphocytes
- 克隆位点
- M17-4
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product ITGAL Primary Antibody
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anti-Integrin alpha-L (ITGAL) (AA 26-306) antibody
ITGAL 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha-L (ITGAL) (AA 26-194) antibodyITGAL 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 5G7C5 unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人 FACS, IP, IHC 宿主: 小鼠 Monoclonal 38 unconjugated
anti-Integrin alpha-L (ITGAL) (AA 201-500) antibodyITGAL 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 小鼠 FACS, IP, IHC (fro) 宿主: 大鼠 Monoclonal M17-4 unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 小鼠 FACS, IP, Func, IHC (fro) 宿主: 大鼠 Monoclonal M17-4 unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-25 unconjugated
anti-Integrin alpha-L (ITGAL) antibodyITGAL 适用: 人 FACS, IP, Func 宿主: 小鼠 Monoclonal MEM-25 unconjugated
anti-Integrin alpha-L (ITGAL) antibody (Biotin)ITGAL 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-25 Biotin
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 31.25 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Cross-linking of the TCR-CD3 complex with CD4, CD8 or LFA-1 induces an anti-apoptotic signal in thymocytes: the signal is canceled by FK506." in: International immunology, Vol. 7, Issue 9, pp. 1387-96, (1996) (PubMed).
: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, (1994) (PubMed).
: "Reduction in the severity of graft-versus-host disease and increased survival in allogenic mice by treatment with monoclonal antibodies to cell adhesion antigens LFA-1 alpha and MALA-2." in: Transplantation, Vol. 52, Issue 5, pp. 842-5, (1991) (PubMed).
: "B cell-associated LFA-1 and T cell-associated ICAM-1 transiently cluster in the area of contact between interacting cells." in: Cellular immunology, Vol. 132, Issue 1, pp. 45-55, (1991) (PubMed).
: "Structure and function of leukocyte integrins." in: Immunological reviews, Vol. 114, pp. 181-217, (1990) (PubMed).
: "Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1." in: The Journal of experimental medicine, Vol. 158, Issue 2, pp. 586-602, (1983) (PubMed).
: "LFA-1 and Lyt-2,3, molecules associated with T lymphocyte-mediated killing; and Mac-1, an LFA-1 homologue associated with complement receptor function." in: Immunological reviews, Vol. 68, pp. 171-95, (1983) (PubMed).
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: "Cross-linking of the TCR-CD3 complex with CD4, CD8 or LFA-1 induces an anti-apoptotic signal in thymocytes: the signal is canceled by FK506." in: International immunology, Vol. 7, Issue 9, pp. 1387-96, (1996) (PubMed).
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- 抗原
- ITGAL (Integrin alpha-L (ITGAL))
- 别名
- CD11a (ITGAL 产品)
- 别名
- CD11A antibody, LFA-1 antibody, LFA1A antibody, (p180) antibody, Cd11a antibody, LFA-1A antibody, Ly-15 antibody, Ly-21 antibody, CD11a antibody, ITGAL antibody, integrin subunit alpha L antibody, integrin alpha L antibody, ITGAL antibody, Itgal antibody
- 背景
- Synonyms: Integrin αL Chain, LFA-1α
- 途径
- Activated T Cell Proliferation, Integrin Complex
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