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Histone 3 抗体 (H3K27me2)

Name: Histone 3 抗体 (H3K27me2)
SKU: ABIN7540290
Availability: OutOfStock

There are 6+ publications for this product available. The 兔多克隆 anti-Histone 3 antibody is suitable to detect Histone 3 in samples from 人. It has been validated for WB, IF, ChIP, ICC, DB 和 ChIP-seq.

Histone H3 dimethyl Lys27 antibody tested by Western blot. (Histone 3 抗体 (H3K27me2))

ChIP

Histone H3 dimethyl Lys27 antibody tested by dot blot analysis. (Histone 3 抗体 (H3K27me2))

Histone H3 dimethyl Lys27 antibody tested by immunofluorescence. (Histone 3 抗体 (H3K27me2))

4 images

(6 references)

产品编号 ABIN7540290

发货至: 中国

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Quick Overview for Histone 3 抗体 (H3K27me2) (ABIN7540290)

抗原

See all Histone 3 (H3) 抗体

Histone 3 (H3) (Histone H3 (H3))

适用

1797
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人

宿主

兔

克隆类型

多克隆

标记

This Histone 3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

Discover our top product H3 Primary Antibody

抗原表位
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H3K27me2

原理

Histone H3K27me2 antibody (pAb)

纯化方法

Unpurified

免疫原

This Histone H3 dimethyl Lys27 antibody was raised against a peptide including dimethyl-lysine 27 of histone H3.
anti-Histone H3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, DB, ChIP-seq 宿主: 小鼠 Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27me) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3R2me2) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me2) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

应用备注

ChIP-Seq: 4-6 µL per ChIP ChIP: 5 - 10 µL per ChIP ICC/IF: 1:200 - 1:500 dilution WB: 1:500 - 1:1,000 dilution

限制

仅限研究用
状态

Liquid

缓冲液

Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

储存条件

-20 °C

储存方法

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

有效期

24 months
Xiao, Shi, Fan, Zhang, Wu, Lan, Minze, Fu, Ghobrial, Liu, Li: "GITR subverts Foxp3(+) Tregs to boost Th9 immunity through regulation of histone acetylation." in: Nature communications, Vol. 6, pp. 8266, (2015) (PubMed).

Herz, Morgan, Gao, Jackson, Rickels, Swanson, Florens, Washburn, Eissenberg, Shilatifard: "Histone H3 lysine-to-methionine mutants as a paradigm to study chromatin signaling." in: Science (New York, N.Y.), Vol. 345, Issue 6200, pp. 1065-70, (2014) (PubMed).

Cie?lik, Hoang, Baranova, Chodaparambil, Kumar, Allison, Xu, Wamsley, Gray, Jones, Mayo, Bekiranov: "Epigenetic coordination of signaling pathways during the epithelial-mesenchymal transition." in: Epigenetics & chromatin, Vol. 6, Issue 1, pp. 28, (2013) (PubMed).

Boulay, Dubuissez, Van Rechem, Forget, Helin, Ayrault, Leprince: "Hypermethylated in cancer 1 (HIC1) recruits polycomb repressive complex 2 (PRC2) to a subset of its target genes through interaction with human polycomb-like (hPCL) proteins." in: The Journal of biological chemistry, Vol. 287, Issue 13, pp. 10509-24, (2012) (PubMed).

Lafos, Kroll, Hohenstatt, Thorpe, Clarenz, Schubert: "Dynamic regulation of H3K27 trimethylation during Arabidopsis differentiation." in: PLoS genetics, Vol. 7, Issue 4, pp. e1002040, (2011) (PubMed).

Qi, Sarkissian, Hu, Wang, Bhattacharjee, Gordon, Gonzales, Lan, Ongusaha, Huarte, Yaghi, Lim, Garcia, Brizuela, Zhao, Roberts, Shi: "Histone H4K20/H3K9 demethylase PHF8 regulates zebrafish brain and craniofacial development." in: Nature, Vol. 466, Issue 7305, pp. 503-7, (2010) (PubMed).
抗原

Histone 3 (H3) (Histone H3 (H3))

别名

Histone H3

背景

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone H3K27 can be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylation can be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.

分子量

17 kDa

基因ID

3020

NCBI登录号

NP_003522