我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

Histone 3 抗体 (H3K27me3)

Name: Histone 3 抗体 (H3K27me3)
SKU: ABIN2668400
Availability: OutOfStock

Cited in 3+ publications. The 小鼠单克隆 anti-Histone 3 antibody (Clone MABI 0323) (ABIN2668400) specifically detects Histone 3 in WB, IF, ChIP, ICC, DB 和 ChIP-seq. The antibody is reactive with 人和小鼠 samples.

Western blot of Histone H3 trimethyl Lys27 antibody. (Histone 3 抗体 (H3K27me3))

Dot blot of Histone H3 trimethyl Lys27 antibody. (Histone 3 抗体 (H3K27me3))

2 images

(3 references)

产品编号 ABIN2668400

发货至: 中国

Contact our Customer Service for availability and price in your country. Contact Info

Our Local Distributor

中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Histone 3 抗体 (H3K27me3) (ABIN2668400)

抗原

See all Histone 3 (H3) 抗体

Histone 3 (H3) (Histone H3 (H3))

适用

1796
1167
1020
48
40
37
36
32
31
23
23
19
9
8
6
6
5
5
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

人, 小鼠

宿主

小鼠

克隆类型

单克隆

标记

This Histone 3 antibody is un-conjugated

应用范围

1449
621
519
424
370
355
251
205
204
188
177
157
121
49
48
42
37
26
25
19
19
7
7
7
4
3
2
2
1
1
1
1

Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

克隆位点

MABI 0323

Discover our top product H3 Primary Antibody

抗原表位
68

59

54

53

53

51

50

49

45

40

39

39

38

36

32

32

32

31

31

31

29

27

27

26

24

23

22

22

21

20

20

19

18

17

16

15

14

14

14

13

13

12

11

11

11

11

10

10

10

10
H3K27me3

原理

Histone H3K27me3 antibody (mAb) (Clone MABI 0323)

纯化方法

Protein G Chromatography

免疫原

This Histone H3 trimethyl Lys27 antibody was raised against a peptide containing trimethyl Lys27 of human Histone H3.

亚型

IgG1
anti-Histone H3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, DB, ChIP-seq 宿主: 小鼠 Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27me) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3R2me2) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me2) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 0.5 - 2 μg/mL dilution DB: 0.5 - 2 μg/mL dilution CUT&Tag: 1 μg per 50 µL reaction

限制

仅限研究用
浓度

0.46 μg/μL

缓冲液

PBS pH 7.5 containing 30 % glycerol. 0.3M NaCl, and 0.035 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

储存条件

-20 °C

储存方法

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

有效期

24 months
Kelsey, Yang, Leung, Minks, Dixon-McDougall, Baldry, Bogutz, Lefebvre, Brown: "Impact of flanking chromosomal sequences on localization and silencing by the human non-coding RNA XIST." in: Genome biology, Vol. 16, pp. 208, (2015) (PubMed).

Herzog, Lempradl, Trupke, Okulski, Altmutter, Ruge, Boidol, Kubicek, Schmauss, Aumayr, Ruf, Pospisilik, Dimond, Senergin, Vargas, Simon, Ringrose: "A strand-specific switch in noncoding transcription switches the function of a Polycomb/Trithorax response element." in: Nature genetics, Vol. 46, Issue 9, pp. 973-81, (2014) (PubMed).

Cooper, Dienstbier, Hassan, Schermelleh, Sharif, Blackledge, De Marco, Elderkin, Koseki, Klose, Heger, Brockdorff: "Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment." in: Cell reports, Vol. 7, Issue 5, pp. 1456-1470, (2014) (PubMed).
抗原

Histone 3 (H3) (Histone H3 (H3))

别名

Histone H3

背景

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.

分子量

17 kDa

基因ID

3020

NCBI登录号

NP_003522