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Histone 3 抗体 (H3K9me1)

Name: Histone 3 抗体 (H3K9me1)
SKU: ABIN2668394
Availability: OutOfStock

Cited in 2+ publications. The 小鼠单克隆 anti-Histone 3 antibody (Clone MABI 0306) (ABIN2668394) specifically detects Histone 3 in WB, ChIP 和 DB. The antibody is reactive with 人 samples.

Histone H3 monomethyl Lys9 antibody tested by Western blot. (Histone 3 抗体 (H3K9me1))

Histone H3 monomethyl Lys9 antibody specificity tested by peptide array analysis. (Histone 3 抗体 (H3K9me1))

PAr

Histone H3 monomethyl Lys9 mAb tested by dot blot analysis. (Histone 3 抗体 (H3K9me1))

3 images

(2 references)

产品编号 ABIN2668394

发货至: 中国

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中国
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Quick Overview for Histone 3 抗体 (H3K9me1) (ABIN2668394)

抗原

See all Histone 3 (H3) 抗体

Histone 3 (H3) (Histone H3 (H3))

适用

1797
1169
1020
48
40
37
36
32
31
23
23
19
9
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1
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1
1
1
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人

宿主

小鼠

克隆类型

单克隆

标记

This Histone 3 antibody is un-conjugated

应用范围

1450
623
519
425
372
355
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205
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188
177
158
121
49
48
42
37
26
25
19
19
7
7
7
4
3
2
2
1
1
1
1

Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

克隆位点

MABI 0306

Discover our top product H3 Primary Antibody

抗原表位
68

59

54

54

53

51

50

49

45

40

39

39

38

36

32

32

32

31

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31

29

27

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10

10
H3K9me1

原理

Histone H3K9me1 antibody (mAb) (Clone MABI 0306)

纯化方法

Protein G Chromatography

免疫原

This Histone H3 monomethyl Lys9 antibody was raised against a synthetic peptide containing monomethyl Lys9 of human histone H3.

亚型

IgG2a
anti-Histone H3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, DB, ChIP-seq 宿主: 小鼠 Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27me) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3R2me2) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me2) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 0.5 - 2 μg/mL dilution

限制

仅限研究用
浓度

0.4 μg/μL

缓冲液

PBS pH 7.5 containing 30 % glycerol and 0.035 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

储存条件

-20 °C

储存方法

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

有效期

24 months
Mitsuda, Shimizu: "Epigenetic Repeat-Induced Gene Silencing in the Chromosomal and Extrachromosomal Contexts in Human Cells." in: PLoS ONE, Vol. 11, Issue 8, pp. e0161288, (2016) (PubMed).

Gregory, Cheung: "Natural variation in the histone demethylase, KDM4C, influences expression levels of specific genes including those that affect cell growth." in: Genome research, Vol. 24, Issue 1, pp. 52-63, (2014) (PubMed).
抗原

Histone 3 (H3) (Histone H3 (H3))

别名

Histone H3

背景

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.

分子量

17 kDa

基因ID

3020

NCBI登录号

NP_003522