Histone 3 抗体 (N-Term)
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北京 101111
Quick Overview for Histone 3 抗体 (N-Term) (ABIN2668390)
抗原
See all Histone 3 (H3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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原理
- Histone H3 antibody (mAb) (Clone MABI 0301)
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纯化方法
- Protein G Chromatography
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免疫原
- This Histone H3 antibody (mAb) was raised against a peptide containing the N-terminus of histone H3.
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亚型
- IgG2b
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anti-Histone H3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K4me) antibodyH3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K27ac) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K9ac) antibodyH3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K36me3) antibodyH3 适用: 人, 小鼠 WB, IF, ChIP, DB, ChIP-seq 宿主: 小鼠 Monoclonal MABI 0333 unconjugated
anti-Histone H3 (H3) (AA 71-136) antibodyH3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K27me) antibodyH3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K4me3) antibodyH3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3R2me2) antibodyH3 适用: 人 WB, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K36me2) antibodyH3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
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应用备注
- ChIP-Seq: 4 μg per ChIP ChIP: 5 - 10 μg per ChIP ICC/IF: 1 μg/mL dilution For Histone H3, we also offer AbFlex Histone H3 Recombinant Antibody (rAb). For details, see Catalog No. 91297.
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限制
- 仅限研究用
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浓度
- 0.54 μg/μL
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缓冲液
- PBS pH 7.5 containing 30 % glycerol. 0.3M NaCl, and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "Acetate Recapturing by Nuclear Acetyl-CoA Synthetase 2 Prevents Loss of Histone Acetylation during Oxygen and Serum Limitation." in: Cell reports, Vol. 18, Issue 3, pp. 647-658, (2017) (PubMed).
: "Histone H3 phosphorylation in GBM: a new rational to guide the use of kinase inhibitors in anti-GBM therapy." in: Theranostics, Vol. 5, Issue 1, pp. 12-22, (2015) (PubMed).
: "L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation." in: Cell communication and signaling : CCS, Vol. 13, pp. 36, (2015) (PubMed).
: "Development of novel cellular histone-binding and chromatin-displacement assays for bromodomain drug discovery." in: Epigenetics & chromatin, Vol. 8, pp. 37, (2015) (PubMed).
: "Chromatin condensation of Xist genomic loci during oogenesis in mice." in: Development (Cambridge, England), Vol. 142, Issue 23, pp. 4049-55, (2015) (PubMed).
: "Paternal heterochromatin formation in human embryos is H3K9/HP1 directed and primed by sperm-derived histone modifications." in: Nature communications, Vol. 5, pp. 5868, (2014) (PubMed).
: "Single-molecule analysis of combinatorial epigenomic states in normal and tumor cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 19, pp. 7772-7, (2013) (PubMed).
: "Regulation of GATA factor expression is distinct between erythroid and mast cell lineages." in: Molecular and cellular biology, Vol. 32, Issue 23, pp. 4742-55, (2012) (PubMed).
: "Ordered arrays of native chromatin molecules for high-resolution imaging and analysis." in: ACS nano, Vol. 6, Issue 9, pp. 7928-34, (2012) (PubMed).
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- Histone 3 (H3) (Histone H3 (H3))
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别名
- Histone H3
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背景
- Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Each histone contains two domains. First, a main globular domain (C-terminal) forming the core of the nucleosome is involved in histone-histone interactions and in binding to the DNA. Secondly, an N-terminal tail is subject to post-translational modifications. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, these modifications play a major role in regulating gene expression.
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分子量
- 17 kDa
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基因ID
- 3020
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NCBI登录号
- NP_003522
抗原
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