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Histone 3 抗体 (H3K56ac)

Name: Histone 3 抗体 (H3K56ac)
SKU: ABIN2668375
Availability: OutOfStock

There are 3+ publications for this product available. The 小鼠单克隆 anti-Histone 3 antibody is suitable to detect Histone 3 in samples from 人, Saccharomyces cerevisiae 和 Schizosaccharomyces pombe. It has been validated for ChIP 和 DB.

Histone H3 acetyl Lys56 pAb tested by dot blot analysis. (Histone 3 抗体 (H3K56ac))

Chromatin IP analysis of Histone H3 acetyl Lys56 antibody. (Histone 3 抗体 (H3K56ac))

ChIP

2 images

(3 references)

产品编号 ABIN2668375

发货至: 中国

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Quick Overview for Histone 3 抗体 (H3K56ac) (ABIN2668375)

抗原

See all Histone 3 (H3) 抗体

Histone 3 (H3) (Histone H3 (H3))

适用

1796
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人, Saccharomyces cerevisiae, Schizosaccharomyces pombe

宿主

小鼠

克隆类型

单克隆

标记

This Histone 3 antibody is un-conjugated

应用范围

1450
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Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

克隆位点

12-1

Discover our top product H3 Primary Antibody

抗原表位
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H3K56ac

原理

Histone H3K56ac antibody (mAb) (Clone 12.1)

纯化方法

Protein A Chromatography

免疫原

This Histone H3 acetyl Lys56 antibody was raised against a peptide containing acetyl-Lys56 of human histone H3.

亚型

IgG1
anti-Histone H3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me) antibody
H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 适用: 人, 小鼠 WB, IF, ChIP, DB, ChIP-seq 宿主: 小鼠 Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27me) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IHC, IF, ChIP, ICC, DB, ChIP-seq, CUT&Tag, CUT&RUN 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3R17me) antibody
H3 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated

应用备注

ChIP: 2 - 10 μg per ChIP DB: 1 μg/mL This antibody has not been validated for use in Western blotting. For a Histone H3K56ac antibody recommended for Western blot, see Catalog No. 39281. This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91127.

限制

仅限研究用
浓度

1 μg/μL

缓冲液

Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

储存条件

-20 °C

储存方法

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

有效期

24 months
Zhang, Li, Rezaeian, Xu, Chou, Jin, Han, Pan, Wang, Long, Zhang, Huang, Tsai, Tsai, Logothetis, Lin: "H3 ubiquitination by NEDD4 regulates H3 acetylation and tumorigenesis." in: Nature communications, Vol. 8, pp. 14799, (2017) (PubMed).

Tessarz, Santos-Rosa, Robson, Sylvestersen, Nelson, Nielsen, Kouzarides: "Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification." in: Nature, Vol. 505, Issue 7484, pp. 564-8, (2014) (PubMed).

Drogaris, Villeneuve, Pomiès, Lee, Bourdeau, Bonneil, Ferbeyre, Verreault, Thibault: "Histone deacetylase inhibitors globally enhance h3/h4 tail acetylation without affecting h3 lysine 56 acetylation." in: Scientific reports, Vol. 2, pp. 220, (2012) (PubMed).
抗原

Histone 3 (H3) (Histone H3 (H3))

别名

Histone H3

背景

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H3 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as CBP/p300. Acetylation of histones is often associated with transcriptional activation. Histone H3 Lys56 acetylation occurs normally during S phase, but disappears in G2. This modification persists in presence of DNA damage and also plays a role in nucleosome assembly. Rtt109 was shown to be the major histone acetyltransferase (HAT) for Lys56 acetylation.

分子量

17 kDa

基因ID

3020

NCBI登录号

NP_003522