APAF1 抗体
-
- 抗原 See all APAF1 抗体
- APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This APAF1 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- 特异性
- This antibody detects endogenous levels of APAF1 protein (region surrounding His324).
- 交叉反应 （详细）
-
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen
- 纯度
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product APAF1 Primary Antibody
-
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 1-250) antibody
APAF1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 31-350) antibodyAPAF1 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 1138-1237) antibodyAPAF1 适用: 人 WB, ELISA, IHC, ICC, FACS, Neut 宿主: 小鼠 Monoclonal 2H9A1 unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 13-80) antibodyAPAF1 适用: 人, 小鼠, 大鼠 ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 1201-1248) antibodyAPAF1 适用: 人, 大鼠, 斑马鱼 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 104-415) antibodyAPAF1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (C-Term) antibodyAPAF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (C-Term) antibodyAPAF1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (AA 1-1205) antibodyAPAF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Apoptotic Peptidase Activating Factor 1 (APAF1) (C-Term) antibodyAPAF1 适用: 人, 小鼠, 大鼠 WB, IHC, ICC 宿主: 小鼠 Monoclonal 2E10 unconjugated
-
- 应用备注
-
ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- 抗原
- APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))
- 别名
- APAF1 (APAF1 产品)
- 别名
- APAF1 antibody, ARK antibody, Apaf-1 antibody, Apaf1 antibody, CG6829 antibody, D-Apaf-1 antibody, DARK antibody, Dapaf-1 antibody, Dapaf-1/HAC-1 antibody, Dark antibody, Dark/Apaf-I antibody, Dark/Dapaf-1/HAC1 antibody, Dark/Hac-1/dApaf-1 antibody, Dark/Hac-1/dApaf1 antibody, Dmel\\CG6829 antibody, Hac-1 antibody, Hac-1/Dark antibody, Hac1 antibody, T1 antibody, anon-53Fa antibody, apaf-1 antibody, apaf1 antibody, arc antibody, ark antibody, dAPAF-1 antibody, dApaf-1 antibody, dApaf-1/DARK/HAC-1 antibody, dApaf1 antibody, dArk antibody, dapaf antibody, dapaf-1 antibody, dapaf-1L antibody, dapaf-1S antibody, dark antibody, dark/dapaf-1/hac-1 antibody, dark/hac-1/dapaf-1 antibody, hac-1 antibody, hac1 antibody, l(2)SH0173 antibody, APAF-1 antibody, CED4 antibody, 6230400I06Rik antibody, Apaf1l antibody, fog antibody, mKIAA0413 antibody, zgc:136543 antibody, Death-associated APAF1-related killer antibody, apoptotic peptidase activating factor 1 L homeolog antibody, apoptotic peptidase activating factor 1 antibody, Dark antibody, apaf1.L antibody, apaf1 antibody, APAF1 antibody, Apaf1 antibody
- 背景
- Studies of apoptosis using the nematode Caenorhabditis elegans as a model system have identified three genes, ced-3, ced-4 and ced-9, as core components in cell death. The Ced-4 protein functions downstream of the apoptotic inhibitor Ced-9 and upstream of Ced-3 to promote cell death. Direct interactions of Ced-4 with Ced-9 and Ced-3 suggest a central role for this protein in the regulation of apoptosis. The mammalian protein AFAP1 contains a domain with homology to Ced-4 and may function in a similar manner by mediating apoptosis downstream of the survival protein Bcl-2 and upstream of the apoptotic inducing caspases. AFAP1 has been shown to bind to cytosolic cytochrome c, and it may mediate the activation of caspase-3 by cytochrome c.Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413
- 分子量
- approx. 38 kDa
- 基因ID
- 317
- NCBI登录号
- NP_001151
- UniProt
- O14727
- 途径
- p53 Pathway, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity
-
