Western Blot: 1-5 μg. Immunoprecipitation: often used in combination with Ig purified anti pp2A/C toimmunoprecipitate heterotrimeric pp2A. Immunocytochemistry: 1/500-1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS pH 7.4 and 0.08 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原
PPP2R1A
(Protein Phosphatase 2, Regulatory Subunit A, alpha (PPP2R1A))
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背景
Protein phosphatase 2A (PP2A) is the most abundant phosphatase specific for serine and threonine phosphorylation in mammalian cells. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit alpha (PPP2R1A) serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory beta subunit.Synonyms: PP2A subunit A PR65-alpha isoform, PP2A subunit A R1-alpha isoform, Protein phosphatase 2A, Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform