TNF alpha 抗体 (Biotin)
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北京 101111
Quick Overview for TNF alpha 抗体 (Biotin) (ABIN264362)
抗原
See all TNF alpha 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin, Tumor necrosis factor ligand superfamily member 2
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纯化方法
- Protein G Chromatography
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纯度
- > 95 % by SDS-PAGE
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免疫原
- Genename: TNF
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亚型
- IgG1
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应用备注
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ELISA: This antibody has been used as a solution phase antibody at 2 μg/mL in a two site ELISAwith unconjugated TNFalpha antibody (Cat. No. ABIN264365). Typically the ELISA has aworking range of 0-10000 pg/mL and a sensitivity of 10 pg/mL with the appropriatestandard. Recommended sample volumes are 25-200 μL. Recovery of human TNFa in human serum is typically >85 % in the ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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缓冲液
- PBS containing 0.08 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. -
有效期
- 12 months
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- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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别名
- Tumor Necrosis Factor (TNF-Alpha)
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背景
- Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2
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UniProt
- P01375
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途径
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
抗原
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